Robo1 (extracellular) Blocking Peptide (#BLP-NR181) is the original antigen used for immunization during Anti-Robo1 (extracellular) Antibody (#ANR-181) generation. The blocking peptide binds and ‘blocks’ Anti-Robo1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
- Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 4), human MDA-MB-231 breast adenocarcinoma cell line lysate (lanes 2 and 5) and human THP-1 monocytic leukemia cell line lysate (lanes 3 and 6):
- Expression of Robo1 in mouse cortexImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Robo1 (extracellular) Antibody (#ANR-181), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Robo1 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with Robo1 (extracellular) Blocking Peptide (#BLP-NR181), suppresses staining. Cell nuclei are stained with DAPI (blue).