Overview
Cat #: BLP-SR011
Type: Synthetic peptide
Form: Lyophilized powder
S1PR1/EDG1 (extracellular) Blocking Peptide (#BLP-SR011) is the original antigen used for immunization during Anti-S1PR1 (EDG1) (extracellular) Antibody (#ASR-011) generation. The blocking peptide binds and ‘blocks’ Anti-S1PR1/EDG1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of (RAEC) rat aortic endothelial cell line lysates (lanes 1 and 3) and A-10 Rat thoracic aorta smooth muscle (lane 2 and 4) cell lysates:1,2. Anti-S1PR1 (EDG1) (extracellular) Antibody (#ASR-011), (1:200).
3,4. Anti-S1PR1 (EDG1) (extracellular) Antibody, preincubated with S1PR1/EDG1 (extracellular) Blocking Peptide (#BLP-SR011). - Expression of Sphingosine 1-phosphate receptor 1 in rat lungImmunohistochemical staining of paraffin embedded rat lung sections using Anti-S1PR1 (EDG1) (extracellular) Antibody (#ASR-011), (1:100). Staining is present in vascular smooth muscle (black arrows) but not in the muscular layer of bronchi (red arrows). Hematoxilin is used as the counterstain.
Properties
Sequence
Accession (Uniprot) Number P21453
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.