S1PR1/EDG1 (extracellular) Blocking Peptide (#BLP-SR011) is the original antigen used for immunization during Anti-S1PR1 (EDG1) (extracellular) Antibody (#ASR-011) generation. The blocking peptide binds and ‘blocks’ Anti-S1PR1/EDG1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of (RAEC) rat aortic endothelial cell line lysates (lanes 1 and 3) and A-10 Rat thoracic aorta smooth muscle (lane 2 and 4) cell lysates:
- Expression of Sphingosine 1-phosphate receptor 1 in rat lungImmunohistochemical staining of paraffin embedded rat lung sections using Anti-S1PR1 (EDG1) (extracellular) Antibody (#ASR-011), (1:100). Staining is present in vascular smooth muscle (black arrows) but not in the muscular layer of bronchi (red arrows). Hematoxilin is used as the counterstain.
- SDYVNYDIIVRHYN(C), corresponding to amino acid residues 17-30 of human S1PR1 (Accession P21453).