Overview
Cat #: BLP-SR012
Type: Synthetic peptide
Form: Lyophilized powder
S1PR2/EDG5 Blocking Peptide (#BLP-SR012) is the original antigen used for immunization during Anti-S1PR2 (EDG5) Antibody (#ASR-012) generation. The blocking peptide binds and ‘blocks’ Anti-S1PR2/EDG5 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat kidney (lanes 1 and 3), rat heart (lanes 2 and 4), human SH-SY5Y brain neuroblastoma (lanes 5 and 7) and Human MS-1 neuroendocrine skin carcinoma cell (lanes 6 and 8) lysates:1,2,5,6. Anti-S1PR2 (EDG5) Antibody (#ASR-012), (1:200).
3,4,7,8. Anti-S1PR2 (EDG5) Antibody, preincubated with S1PR2/EDG5 Blocking Peptide (#BLP-SR012). - Expression of Sphingosine 1-phosphate receptor 2 in rat lungImmunohistochemical staining of paraffin embedded rat lung sections using Anti-S1PR2 (EDG5) Antibody (#ASR-012), (1:100). S1PR2 is expressed in respiratory epithelium (red arrows). Hematoxilin is used as the counterstain.
Properties
Sequence
Accession (Uniprot) Number P52592
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.