Semaphorin 3A/SEMA3A Blocking Peptide (#BLP-SR051) is the original antigen used for immunization during Anti-Semaphorin 3A (SEMA3A) Antibody (#ASR-051) generation. The blocking peptide binds and ‘blocks’ Anti-Semaphorin 3A/SEMA3A primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
- Western blot analysis of human THP-1 acute monocytic leukemia cell lysate (lanes 1 and 3) and human MDA-231 breast adenocarcinoma cell lysate (lanes 2 and 4):
- (C)KRDRKQRRQRPGH, corresponding to amino acid residues 728-740 of rat SEMA3A (Accession Q63548).