SLC17A9 Blocking Peptide (#BLP-NT085) is the original antigen used for immunization during Anti-SLC17A9 Antibody (#ANT-085) generation. The blocking peptide binds and ‘blocks’ Anti-SLC17A9 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
- Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
- Expression of VNUT (SLC17A9) in rat hypothalamusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC17A9 Antibody (#ANT-085), (1:2000), followed by goat anti-rabbit-AlexaFluor-488. A. VNUT immunoreactivity (green) in the paraventricular nucleus (PVN) of the hypothalamus is detected in neurons (vertical arrows) and axonal processes (horizontal arrows). 3V = third ventricle. B. VNUT staining (green) in the supra-optic nucleus (SON) of the hypothalamus is observed in neurons (vertical arrows) and axonal processes (horizontal arrows). OT = Optic tract. Cell nuclei are stained with DAPI (blue).
- (C)SQKVQESERAFTYS, corresponding to amino acid residues 155-168 of mouse VNUT (Accession Q8VCL5).