SLC25A12 Blocking Peptide (#BLP-NT112) is the original antigen used for immunization during Anti-SLC25A12 Antibody (#ANT-112) generation. The blocking peptide binds and ‘blocks’ Anti-SLC25A12 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat skeletal muscle lysate (lanes 1 and 4, 1:600), mouse brain membrane (lanes 2 and 5) and human OVCAR3 ovarian adenocarcinoma cell line lysate (lanes 3 and 6):
- Expression of SLC25A12 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC25A12 Antibody (#ANT-112), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal dentate gyrus region, showed immunoreactivity (green) in neurons of the hilus (H, arrows). B. Pre-incubation of the antibody with SLC25A12 Blocking Peptide (#BLP-NT112), suppressed staining. Cell nuclei are stained with DAPI (blue).
G = granule layer.
- (C)KDKSKSGMISGLD, corresponding to amino acid residues 169-181 of mouse SLC25A12 (Accession Q8BH59).