SLC47A1 Blocking Peptide (#BLP-NT131) is the original antigen used for immunization during Anti-SLC47A1 Antibody (#ANT-131) generation. The blocking peptide binds and ‘blocks’ Anti-SLC47A1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat kidney (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat testis (lanes 3 and 6):
- Expression of SLC47A1 (MATE1) in rat parietal cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC47A1 Antibody (#ANT-131), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. SLC47A1 (MATE1) immunoreactivity (green) appears in pyramidal neurons in the parietal cortex (arrows). B. Pre-incubation of the antibody with SLC47A1 Blocking Peptide (#BLP-NT131), suppressed staining. Cell nuclei are stained with DAPI (blue).
- (C)ESHGEIMMTDLEKK, corresponding to amino acid residues 496-509 of mouse SLC47A1 (Accession Q8K0H1).