STIM2 Blocking Peptide (#BLP-CC064) is the original antigen used for immunization during Anti-STIM2 Antibody (#ACC-064) generation. The blocking peptide binds and ‘blocks’ Anti-STIM2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain (lanes 1, 3) and rat RBL basophilic leukemia cell (lanes 2, 4) lysates:
- Expression of STIM2 in rat lungImmunohistochemical staining of paraffin embedded rat lung sections using Anti-STIM2 Antibody (#ACC-064), (1:100). Staining is present in the respiratory epithelium of the bronchiole (red) as well as in the pneumonocytes of the alveolar wall (blue). Color reaction was obtained with SuperPicture HRP-conjugated polymer (Zymed) followed by DAB. Hematoxilin is used as the counterstain.
- SAEKQWEVPDTASEC, corresponding to amino acid residues 583-597 of human STIM2 (Accession Q9P246).