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Anti-STIM2 Antibody

Stromal interaction molecule 2

Cat #: ACC-064
Alternative Name Stromal interaction molecule 2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide SAEKQWEVPDTASEC, corresponding to amino acid residues 583-597 of human STIM2 (Accession Q9P246). Intracellular, C-terminus.
Accession (Uniprot) Number Q9P246
Gene ID 57620
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse, bovine - identical.
RRID AB_2040218.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat brain (lanes 1, 3) and RBL cell (lanes 2, 4) lysates:
    Western blot analysis of rat brain (lanes 1, 3) and RBL cell (lanes 2, 4) lysates:
    1. Anti-STIM2 Antibody (#ACC-064), (1:200).
    2. Anti-STIM2 Antibody, preincubated with STIM2 Blocking Peptide (#BLP-CC064).
Immunohistochemistry
  • Expression of STIM2 in rat lung
    Expression of STIM2 in rat lung
    Immunohistochemical staining of paraffin embedded rat lung sections using Anti-STIM2 Antibody (#ACC-064), (1:100). Staining is present in the respiratory epithelium of the bronchiole (red) as well as in the pneumonocytes of the alveolar wall (blue). Color reaction was obtained with SuperPicture HRP-conjugated polymer (Zymed) followed by DAB. Hematoxilin is used as the counterstain.
Immunocytochemistry
  • Expression of STIM2 in RBL cells
    Expression of STIM2 in RBL cells
    Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat basophilic leukemia (RBL) cells.
    A,D. Cells stained with Anti-STIM2 Antibody (#ACC-064), (1:100) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody.
    B,E. Nuclear staining of cells using the cell-permeable dye Hoechst 33342.
    C. Merged image of panels A and B
    F. Merged image of panels D and E.
References
  1. Eisner, D.A. et al. (2005) Exp. Physiol. 90, 3.
  2. Chakrabarti, R. and Chakrabarti, R. (2006) J. Cell. Biochem. 99, 1503.
  3. Feske, S. et al. (2006) Nature 441, 179.
  4. Pickett, J. (2006) Nat. Rev. Mol. Cell Biol. 7, 794.
  5. Dziadek, M.A. and Johnstone, L.S. (2007) Cell Calcium 42, 123.
  6. Parvez, S. et al. (2007) FASEB J. 22, 752.
  7. Brandman, O. et al. (2007) Cell 131, 1327.
  8. Soboloff, J. et al. (2006) Curr. Biol. 16, 1465.
Scientific background

Cytosolic Ca2+ has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The mechanism controlling the influx of intracellular Ca2+ either by calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores has lately become the focus of intense research.

Recently, several key players of the store-operated complex have been identified: the Orai family consists of three members: Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2.3 Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum (ER) Ca2+ sensor.3,4

While STIM1 appears to be localized intracellularly at the ER membrane and at a much lower extent on the cell surface of several cell types, STIM2 appears to be localized solely at the ER membrane.5  The function of STIM2 still remains elusive and several models have been suggested.6-8

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title:

Anti-STIM2 Antibody
Expression of STIM2 in mouse cortical neurons and human SH-SY5Y cells.Western blot analysis of mouse cortical neuron lysates and human SH-SY5Y cell lysates using Anti-STIM2 Antibody (#ACC-064). Actin is used as a loading control.Adapted from Gonzalez-Sanchez, P. et al. (2017) Front. Cell. Neurosci. 11, 363. with permission of Frontiers.

Last update: 11/04/2021

Anti-STIM2 Antibody (#ACC-064) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize STIM2 from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Mouse cortical neuron lysates and human SH-SY5Y cell lysates.
    Gonzalez-Sanchez, P. et al. (2017) Front. Cell. Neurosci. 11, 363.
  2. Rat neonatal ventricular cardiomyocytes (1:200).
    Sabourin, J. et al. (2016) J. Biol. Chem. 291, 13394.
  3. Rat cortical neuron cell lysate.
    Gruszczynska-Biegala, J. et al. (2011) PLoS ONE 6, e19285.
Immunohistochemistry citations
  1. Mouse brain sections (1:500).
    Kikuta, S. et al. (2019) Front. Cell. Neurosci. 13, 547.
Immunofluorescence citations
  1. Mouse brain sections (1:500).
    Kikuta, S. et al. (2019) Front. Cell. Neurosci. 13, 547.
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