Overview
Type: Synthetic peptide
Form: Lyophilized powder
Synapsin II/SYN2 Blocking Peptide (#BLP-NR015) is the original antigen used for immunization during Anti-Synapsin II (SYN2) Antibody (#ANR-015) generation. The blocking peptide binds and ‘blocks’ Anti-Synapsin II/SYN2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-Synapsin II (SYN2) Antibody (#ANR-015), (1:200).
3,4. Anti-Synapsin II (SYN2) Antibody, preincubated with Synapsin II/SYN2 Blocking Peptide (#BLP-NR015).
Expression of Synapsin-2 in mouse ventral striatum and rat hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Synapsin II (SYN2) Antibody (#ANR-015), (1:200), followed by goat-anti-rabbit-AlexaFluor-488 (green staining). A. Synapsin-2 staining in the mouse ventral striatum region shows dense patches of immunoreactivity within the islands of Calleja (IC) (horizontal arrow) that are delineated by DAPI-positive granule cells (in blue and marked by a vertical arrow). B. Synapsin-2 staining in the rat hippocampal dentate gyrus is detected in the dentate hilus (H) but not in the granule layer (G). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)SGTLKEPDSSKTP, corresponding to amino acid residues 438-450 of rat Synapsin-2 (Accession Q63537).
Accession (Uniprot) Number Q63537
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
