Cat #: BLP-NR015
Type: Synthetic peptide
Form: Lyophilized powder
Synapsin II/SYN2 Blocking Peptide (#BLP-NR015) is the original antigen used for immunization during Anti-Synapsin II (SYN2) Antibody (#ANR-015) generation. The blocking peptide binds and ‘blocks’ Anti-Synapsin II/SYN2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
WB-western blot, IHC-Immunohistochemistry
For research purposes only, not for human use
Demonstration of Pre-adsorption control
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-Synapsin II (SYN2) Antibody (#ANR-015), (1:200).
3,4. Anti-Synapsin II (SYN2) Antibody, preincubated with Synapsin II/SYN2 Blocking Peptide (#BLP-NR015).
- Expression of Synapsin-2 in mouse ventral striatum and rat hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Synapsin II (SYN2) Antibody (#ANR-015), (1:200), followed by goat-anti-rabbit-AlexaFluor-488 (green staining). A. Synapsin-2 staining in the mouse ventral striatum region shows dense patches of immunoreactivity within the islands of Calleja (IC) (horizontal arrow) that are delineated by DAPI-positive granule cells (in blue and marked by a vertical arrow). B. Synapsin-2 staining in the rat hippocampal dentate gyrus is detected in the dentate hilus (H) but not in the granule layer (G). Cell nuclei are stained with DAPI (blue).
- (C)SGTLKEPDSSKTP, corresponding to amino acid residues 438-450 of rat Synapsin-2 (Accession Q63537).
Accession (Uniprot) Number Q63537
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.