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3, 4. Anti-Synapsin-2 Antibody, preincubated with the control peptide antigen.
Synapsins are a family of ten homologous neuronal phosphoproteins evolutionarily conserved in invertebrate and vertebrate organisms. Synapsins appear to be crucial determinants for the fine-tuning of synaptic transmission and for synaptic remodeling. This is particularly evident in several paradigms of short-term plasticity (STP), which are specifically affected by mutations or malfunctioning of synapsin proteins. Notably, knockout of synapsin-2 in mice causes fully generalized forebrain seizures from the age of 2 months1.
All Synapsins, including Synapsin-2, are composed of a highly homologous N-terminal region, and a more variable C-terminus. The N-terminal homologous region can be divided in three domains, namely domain A, B and C, which, except for domain B, are highly conserved across isoforms and species. Domain A is contains the phosphorylation site for PKA and CaMKI. Domain B, rich in small amino acids, is considered as a linker region connecting domain A to domain C. Domain C is a large region of about 300 amino acids, containing both hydrophobic and highly charged sequences with the potential to assume α-helix and β-sheet conformations. It shows extensive homology amongst the various members of the family, and mediates the core functions of synapsins1.
Species reactivity key:
Immuno-colocalization of Synapsin-2 and GABA(A) α1 Receptor in rat striatum.Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using rabbit Anti-Synapsin-2 Antibody (#ANR-015), (1:300) and Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGP-083), (1:600). A. Synapsin-2 staining (red) appears in Islands of Calleja (IC), (arrow). B. GABA(A) α1 Receptor (green) is also detected in Islands of Calleja and in adjacent neurons. C. Merge of the two images demonstrates colocalization in IC while adjacent GABA(A) α1 positive neurons do not express Synapsin-2. Cell nuclei are stained with DAPI (blue).
Anti-Synapsin-2 Antibody (#ANR-015) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize Synapsin-2 from mouse, human, and rat samples. This antibody will recognize both canonical isoforms (also known as Synapsin IIa) and the shorter isoform (Synapsin-IIb).
- Anti-Synaptotagmin-I Antibody (#ANR-003)
- Anti-Synaptophysin Antibody (#ANR-013)
- Anti-Synapsin-1 Antibody (#ANR-014)
- Anti-RIM1 Antibody (#AIP-014)
- Anti-VGLUT1 Antibody (#AGC-035)
- Anti-Vesicular GABA Transporter Antibody (#AGT-005)
- Anti-CPLX1/2 Antibody (#ANR-011)
- Presynaptic Marker Antibody Explorer Kit (#AK-235)