Anti-Synapsin-2 Antibody

Synapsin II, SYN2
    Cat #: ANR-015
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: m, r
    May also work in: h
    Immunogen
    Peptide (C)SGTLKEPDSSKTP, corresponding to amino acid residues 438-450 of rat Synapsin-2 (Accession Q63537). Intracellular, region G.
    Accession (Uniprot) Number Q63537
    Gene ID 29179
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – identical; human- 12/13 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Specificity This antibody will recognize both canonical isoforms (also known as Synapsin IIa) and the shorter isoform (Synapsin-IIb).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip
    Western blot
    Western blot analysis of rat (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): 
    1, 2. Anti-Synapsin-2 Antibody (#ANR-015), (1:200).
    3, 4. Anti-Synapsin-2 Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of Synapsin-2 in mouse ventral striatum and rat hippocampus
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Synapsin-2 Antibody (#ANR-015), (1:200), followed by goat-anti-rabbit-AlexaFluor-488 (green staining). A. Synapsin-2 staining in the mouse ventral striatum region, shows dense patches of immunoreactivity within the islands of Calleja (IC) (horizontal arrow), that are delineated by DAPI-positive granule cells (in blue and marked by a vertical arrow). B. Synapsin-2 staining in the rat hippocampal dentate gyrus, is detected in the dentate hilus (H), but not in the granule layer (G). Cell nuclei are stained with DAPI (blue).
    References
    1. Etholm, L. et al. (2012) Epilepsy Res. 99, 252.
    2. Cesca, F. et al. (2010) Prog. Neurobiol. 91, 313.
    Scientific background

    Synapsins are a family of ten homologous neuronal phosphoproteins evolutionarily conserved in invertebrate and vertebrate organisms. Synapsins appear to be crucial determinants for the fine-tuning of synaptic transmission and for synaptic remodeling. This is particularly evident in several paradigms of short-term plasticity (STP), which are specifically affected by mutations or malfunctioning of synapsin proteins. Notably, knockout of synapsin-2 in mice causes fully generalized forebrain seizures from the age of 2 months1.

    All Synapsins, including Synapsin-2, are composed of a highly homologous N-terminal region, and a more variable C-terminus. The N-terminal homologous region can be divided in three domains, namely domain A, B and C, which, except for domain B, are highly conserved across isoforms and species. Domain A is contains the phosphorylation site for PKA and CaMKI. Domain B, rich in small amino acids, is considered as a linker region connecting domain A to domain C. Domain C is a large region of about 300 amino acids, containing both hydrophobic and highly charged sequences with the potential to assume α-helix and β-sheet conformations. It shows extensive homology amongst the various members of the family, and mediates the core functions of synapsins1.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Immuno-colocalization of Synapsin-2 and GABA(A) α1 Receptor in rat striatum.Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using rabbit Anti-Synapsin-2 Antibody (#ANR-015), (1:300) and Guinea pig Anti-GABA(A) α1 Receptor (extracellular) Antibody (#AGP-083), (1:600). A. Synapsin-2 staining (red) appears in Islands of Calleja (IC), (arrow). B. GABA(A) α1 Receptor (green) is also detected in Islands of Calleja and in adjacent neurons. C. Merge of the two images demonstrates colocalization in IC while adjacent GABA(A) α1 positive neurons do not express Synapsin-2. Cell nuclei are stained with DAPI (blue).

    Last update: 29/10/2018

    Anti-Synapsin-2 Antibody (#ANR-015) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize Synapsin-2 from mouse, human, and rat samples. This antibody will recognize both canonical isoforms (also known as Synapsin IIa) and the shorter isoform (Synapsin-IIb).

    For research purposes only, not for human use