Overview
Type: Synthetic peptide
Form: Lyophilized powder
Synaptotagmin-1/SYT1 Blocking Peptide (#BLP-NR003) is the original antigen used for immunization during Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003) generation. The blocking peptide binds and ‘blocks’ Anti-Synaptotagmin-1/SYT1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003), (1:400).
3,4. Anti-Synaptotagmin-1 (SYT1) Antibody, preincubated with Synaptotagmin-1/SYT1 Blocking Peptide (#BLP-NR003).
Expression of Synaptotagmin-I in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003), (1:400), followed by anti-rabbit-Alexa-488 antibody. Synaptotagmin-I staining (green) appears in the hilus of the hippocampal dentate gyrus. Nuclei are stained with DAPI (blue).
Western blot analysis of rat brain lysates:1. Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003-GP), (1:400).
2. Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003-GP), preincubated with Synaptotagmin-1 (SYT1) Blocking Peptide (#BLP-NR003).
Western blot analysis of mouse brain lysates:1. Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003-GP), (1:200).
2. Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003-GP), preincubated with Synaptotagmin-1 (SYT1) Blocking Peptide (#BLP-NR003).
Expression of Synaptotagmin-1 in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003-GP), (1:1000), followed by goat anti-guinea pig - Alexa Fluor-594. A. Synaptotagmin-1 immunoreactivity (red) appears in the granule layer (vertical arrows) and in the molecular layer (horizontal arrows). B. Pre-incubation of the antibody with Synaptotagmin-1 (SYT1) Blocking Peptide (#BLP-NR003), suppressed staining. Cell nuclei are stained with DAPI (blue). M = molecular layer, P = purkinje layer, G = granule layer.
Expression of Synaptotagmin-1 in rat spinal cord.Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003-GP), (1:1000), followed by goat anti-guinea pig - Alexa Fluor-594. A. Staining in the spinal dorsal horn region, showed Synaptotagmin-1 immunoreactivity (red) in axonal outlines in the superficial layer of the dorsal horn (arrows). B. Pre-incubation of the antibody with Synaptotagmin-1 (SYT1) Blocking Peptide (#BLP-NR003), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- MVSASHPEALAAPVTTVAT(C), corresponding to amino acid residues 1-19 of rat synaptotagmin 1 (Accession P21107).
Accession (Uniprot) Number P21107
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
