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Anti-Synaptotagmin-1 (SYT1) Antibody

Synaptotagmin I, SytI, p65, SVP65

Cat #: ANR-003
Alternative Name Synaptotagmin I, SytI, p65, SVP65
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide MVSASHPEALAAPVTTVAT(C), corresponding to amino acid residues 1-19 of rat synaptotagmin 1 (Accession P21107). Intravesicular, N-terminus.
Accession (Uniprot) Number P21107
Gene ID 25716
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 18/19 amino acid residues identical; human, bovine - 17/20 amino acid residues identical; chicken - 15/19 amino acid residues identical.
RRID AB_2040222.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.2 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    1,2. Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003), (1:400).
    3,4. Anti-Synaptotagmin-1 (SYT1) Antibody, preincubated with Synaptotagmin-1/SYT1 Blocking Peptide (#BLP-NR003).
Immunohistochemistry
  • Expression of Synaptotagmin-I in mouse hippocampus
    Expression of Synaptotagmin-I in mouse hippocampus
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003), (1:400), followed by anti-rabbit-Alexa-488 antibody. Synaptotagmin-I staining (green) appears in the hilus of the hippocampal dentate gyrus. Nuclei are stained with DAPI (blue).
References
  1. Südhof, T.C. (2002) J. Biol. Chem. 277, 7629.
  2. Malsam, J. et al. (2008) Cell. Mol. Life Sci. 65, 2814.
  3. Meir, A. et al. (1999) Physiol. Rev. 79, 1019.
Scientific background

Synaptotagmin 1 is a member of the Ca2+-binding synaptotagmin family of proteins that includes 16 members in vertebrates and is involved in regulated exocytosis and trafficking1,2.

Synaptotagmins are type I membrane proteins located in intracellular vesicles and consist of a short N-terminal domain located in the vesicle lumen, a transmembrane domain and a cytosolic domain containing two Ca2+ binding domains1,2.

Synaptotagmins are usually localized to distinct secretory vesicles and control their Ca2+-dependent fusion. Synaptotagmin I is predominantly expressed in neuronal cells and is an abundant constituent of synaptic vesicles1,2.

Synaptotagmin I has a relatively low Ca2+ affinity and is able to bind Ca2+ mostly in the vicinity of Ca2+ channels pores. Indeed, Synaptotagmin I has been demonstrated to directly interact with selected Ca2+ channels. Synaptotagmin I also binds to the t-SNARE protein Syntaxin 1 in a Ca2+-dependent manner thus forming the prototypical Ca2+-dependent fast synaptic vesicle exocytosis protein complex that also includes SNAP-25 and VAMP1,2,3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Alomone Labs is pleased to offer a highly specific antibody directed against the intravesicular N-terminal domain of rat synaptotagmin 1. Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003) can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize synaptotagmin 1 from rat, mouse and human samples.

For research purposes only, not for human use

Applications

Citations

Citations
Immunocytochemistry citations
  1. Rat PC12 cells.
    Peixoto-Neves, D. et al. (2018) Biochem. Biophys. Res. Commun. 506, 709.
More product citations
  1. Zhao, X.S. et al. (2001) EMBO J. 20, 2680.
  2. Shin, D.M. et al. (2000) J. Cell Biol. 150, 1101.
  3. Barbosa, J. et al. (1999) J. Neurochem. 73, 1881.

Specifications

Scientific Background

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