- Peptide MVSASHPEALAAPVTTVAT(C), corresponding to amino acid residues 1-19 of rat synaptotagmin 1 (Accession P21107). Intravesicular, N-terminus.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003), (1:400).
3,4. Anti-Synaptotagmin-1 (SYT1) Antibody, preincubated with Synaptotagmin-1/SYT1 Blocking Peptide (#BLP-NR003).
- Expression of Synaptotagmin-I in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003), (1:400), followed by anti-rabbit-Alexa-488 antibody. Synaptotagmin-I staining (green) appears in the hilus of the hippocampal dentate gyrus. Nuclei are stained with DAPI (blue).
- Südhof, T.C. (2002) J. Biol. Chem. 277, 7629.
- Malsam, J. et al. (2008) Cell. Mol. Life Sci. 65, 2814.
- Meir, A. et al. (1999) Physiol. Rev. 79, 1019.
Synaptotagmin 1 is a member of the Ca2+-binding synaptotagmin family of proteins that includes 16 members in vertebrates and is involved in regulated exocytosis and trafficking1,2.
Synaptotagmins are type I membrane proteins located in intracellular vesicles and consist of a short N-terminal domain located in the vesicle lumen, a transmembrane domain and a cytosolic domain containing two Ca2+ binding domains1,2.
Synaptotagmins are usually localized to distinct secretory vesicles and control their Ca2+-dependent fusion. Synaptotagmin I is predominantly expressed in neuronal cells and is an abundant constituent of synaptic vesicles1,2.
Synaptotagmin I has a relatively low Ca2+ affinity and is able to bind Ca2+ mostly in the vicinity of Ca2+ channels pores. Indeed, Synaptotagmin I has been demonstrated to directly interact with selected Ca2+ channels. Synaptotagmin I also binds to the t-SNARE protein Syntaxin 1 in a Ca2+-dependent manner thus forming the prototypical Ca2+-dependent fast synaptic vesicle exocytosis protein complex that also includes SNAP-25 and VAMP1,2,3.
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against the intravesicular N-terminal domain of rat synaptotagmin 1. Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003) can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize synaptotagmin 1 from rat, mouse and human samples.
- Rat PC12 cells.
Peixoto-Neves, D. et al. (2018) Biochem. Biophys. Res. Commun. 506, 709.
- Zhao, X.S. et al. (2001) EMBO J. 20, 2680.
- Shin, D.M. et al. (2000) J. Cell Biol. 150, 1101.
- Barbosa, J. et al. (1999) J. Neurochem. 73, 1881.