Anti-Synaptotagmin-1 (SYT1) Antibody

Synaptotagmin I, SytI, p65, SVP65
    Cat #: ANR-003
    Alternative Name Synaptotagmin I, SytI, p65, SVP65
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
      • Peptide MVSASHPEALAAPVTTVAT(C), corresponding to amino acid residues 1-19 of rat synaptotagmin 1 (Accession P21107). Intravesicular, N-terminus.
        Anti-Synaptotagmin-1 (SYT1) Antibody
    Accession (Uniprot) Number P21107
    Gene ID 25716
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 18/19 amino acid residues identical; human, bovine - 17/20 amino acid residues identical; chicken - 15/19 amino acid residues identical.
    RRID AB_2040222.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.2 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*
      • Anti-Synaptotagmin-1 (SYT1) Antibody
        Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
        1,2. Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003), (1:400).
        3,4. Anti-Synaptotagmin-1 (SYT1) Antibody, preincubated with the control peptide antigen.
      • Anti-Synaptotagmin-1 (SYT1) Antibody
        Expression of Synaptotagmin-I in mouse hippocampus
        Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003), (1:400), followed by anti-rabbit-Alexa-488 antibody. Synaptotagmin-I staining (green) appears in the hilus of the hippocampal dentate gyrus. Nuclei are stained with DAPI (blue).
        Rat brain sections.
    References
    • 1. Südhof, T.C. (2002) J. Biol. Chem. 277, 7629.
    • 2. Malsam, J. et al. (2008) Cell. Mol. Life Sci. 65, 2814.
    • 3. Meir, A. et al. (1999) Physiol. Rev. 79, 1019.
      • Synaptotagmin 1 is a member of the Ca2+-binding synaptotagmin family of proteins that includes 16 members in vertebrates and is involved in regulated exocytosis and trafficking1,2.

        Synaptotagmins are type I membrane proteins located in intracellular vesicles and consist of a short N-terminal domain located in the vesicle lumen, a transmembrane domain and a cytosolic domain containing two Ca2+ binding domains1,2.

        Synaptotagmins are usually localized to distinct secretory vesicles and control their Ca2+-dependent fusion. Synaptotagmin I is predominantly expressed in neuronal cells and is an abundant constituent of synaptic vesicles1,2.

        Synaptotagmin I has a relatively low Ca2+ affinity and is able to bind Ca2+ mostly in the vicinity of Ca2+ channels pores. Indeed, Synaptotagmin I has been demonstrated to directly interact with selected Ca2+ channels. Synaptotagmin I also binds to the t-SNARE protein Syntaxin 1 in a Ca2+-dependent manner thus forming the prototypical Ca2+-dependent fast synaptic vesicle exocytosis protein complex that also includes SNAP-25 and VAMP1,2,3.


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    Last update: 11/11/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against the intravesicular N-terminal domain of rat synaptotagmin 1. Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003) can be used in western blot and immunohistochemistry applications. It has been designed to recognize synaptotagmin 1 from rat, mouse and human samples.

    For research purposes only, not for human use

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