SynDIG1 Blocking Peptide (#BLP-GC049) is the original antigen used for immunization during Anti-SynDIG1 Antibody (#AGC-049) generation. The blocking peptide binds and ‘blocks’ Anti-SynDIG1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
- Western blot analysis of human SH-SY5Y neuroblastoma cell line:
- (C)RSPTKDSLEYPDGK, corresponding to amino acid residues 119-132 of rat SynDIG1 (Accession Q58DZ9).