Thromboxane A2 Receptor Blocking Peptide (#BLP-PR069) is the original antigen used for immunization during Anti-Thromboxane A2 Receptor Antibody (#APR-069) generation. The blocking peptide binds and ‘blocks’ Anti-Thromboxane A2 Receptor primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
- Western blot analysis of human THP-1 monocytic leukemia (lanes 1 and 3) and human MEG-01 megakaryoblastic leukemia (lanes 2 and 4) cell line lysates:
- Expression of thromboxane A2 receptor in mouse cortex and hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Thromboxane A2 Receptor Antibody (#APR-069), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. TBXA2R immunoreactivity (green) is observed in mouse parietal cortex neurons (arrows). B. TBXA2R immunoreactivity (green) is detected in neurons (horizontal arrows) of mouse hippocampal CA3 pyramidal layer (P). Cell nuclei are stained with DAPI (blue).
- (C)HAREATQRPRD(S)EVE, corresponding to amino acid residues 225-239 of rat TBXA2R (Accession P34978).