Overview
Type: Synthetic peptide
Form: Lyophilized powder
TMC6/EVER1 Blocking Peptide (#BLP-TC006) is the original antigen used for immunization during Anti-TMC6 (EVER1) Antibody (#ATC-006) generation. The blocking peptide binds and ‘blocks’ Anti-TMC6/EVER1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat prostate (lanes 1 and 4), testis (lanes 2 and 5) and placenta (lanes 3 and 6):1-3. Anti-TMC6 (EVER1) Antibody (#ATC-006), (1:800).
4-6. Anti-TMC6 (EVER1) Antibody, preincubated with TMC6/EVER1 Blocking Peptide (#BLP-TC006).
Properties
Sequence
- CGPFRTLDTMYEAGR, corresponding to amino acid residues 681-695 of human TMC6 (Accession Q7Z403).
Accession (Uniprot) Number Q7Z403
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
