TMEM119 (extracellular) Blocking Peptide (#BLP-NR175) is the original antigen used for immunization during Anti-TMEM119 (extracellular) Antibody (#ANR-175) generation. The blocking peptide binds and ‘blocks’ Anti-TMEM119 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
- Western blot analysis mouse BV-2 microglia cell lysate:
- Peptide (C)GTPAFSPTPERPQPT, corresponding to amino acid residues 53 - 67 of mouse TMEM119 (Accession Q8R138).