TPCN2 Blocking Peptide (#BLP-CC072) is the original antigen used for immunization during Anti-TPCN2 Antibody (#ACC-072) generation. The blocking peptide binds and ‘blocks’ Anti-TPCN2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse kidney lysate (lanes 1 and 4), rat lung membrane (lanes 2 and 5) and human embryonic Kidney 293 cell lysate (lanes 3 and 6):
- Expression of Two pore calcium channel protein 2 in rat cerebellumImmunohistochemical staining of Two pore calcium channel protein 2 in rat cerebellum using Anti-TPCN2 Antibody (#ACC-072). A. TPCN2 positive cells (green) appear in the granule cell layer (G) of the cerebellum (arrows). B. The extent of the granule layer is demonstrated with the counterstain DAPI (blue).
- KKTLKSIRW(S)LPE(C), corresponding to amino acid residues 187-199 of mouse TPCN2 with replacement of amino acid 192 with serine (S) (Accession Q8BWC0).