Overview
Type: Synthetic peptide
Form: Lyophilized powder
TPCN2 Blocking Peptide (#BLP-CC072) is the original antigen used for immunization during Anti-TPCN2 Antibody (#ACC-072) generation. The blocking peptide binds and ‘blocks’ Anti-TPCN2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse kidney lysate (lanes 1 and 4), rat lung membrane (lanes 2 and 5) and human embryonic Kidney 293 cell lysate (lanes 3 and 6):1-3. Anti-TPCN2 Antibody (#ACC-072), (1:200).
4-6. Anti-TPCN2 Antibody, preincubated with TPCN2 Blocking Peptide (#BLP-CC072).
Expression of Two pore calcium channel protein 2 in rat cerebellumImmunohistochemical staining of Two pore calcium channel protein 2 in rat cerebellum using Anti-TPCN2 Antibody (#ACC-072). A. TPCN2 positive cells (green) appear in the granule cell layer (G) of the cerebellum (arrows). B. The extent of the granule layer is demonstrated with the counterstain DAPI (blue).
Properties
Sequence
- KKTLKSIRW(S)LPE(C), corresponding to amino acid residues 187-199 of mouse TPCN2 with replacement of amino acid 192 with serine (S) (Accession Q8BWC0).
Accession (Uniprot) Number Q8BWC0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
