TRPC1 Blocking Peptide (#BLP-CC010) is the original antigen used for immunization during Anti-TRPC1 Antibody (#ACC-010) generation. The blocking peptide binds and ‘blocks’ Anti-TRPC1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:
- Expression of TRPC1 in mouse cerebellumImmunohistochemical staining of mouse cerebellum frozen sections using Anti-TRPC1 Antibody (#ACC-010). A. TRPC1 (red) appears in Purkinje cells (arrows) and in the molecular (Mol) and granule (Gran) layers. B. Staining with mouse anti-parvalbumin (PV) in the same brain section. C. Confocal merge of TRPC1 and PV demonstrates partial co-localization in the Purkinje and the molecular layers.
- QLYDKGYTSKEQKDC, corresponding to amino acid residues 557-571 of human TRPC1 (Accession P48995).