TRPM2 (extracellular) Blocking Peptide (#BLP-CC128) is the original antigen used for immunization during Anti-TRPM2 (extracellular) Antibody (#ACC-128) generation. The blocking peptide binds and ‘blocks’ Anti-TRPM2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
- Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human HL-60 promyelocytic leukemia cell line lysate (lanes 2 and 4):
- Expression of TRPM2 in rat substantia nigraImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-TRPM2 (extracellular) Antibody (#ACC-128), followed by goat anti-rabbit-AlexaFluor-488. TRPM2 immunoreactivity (green) appears in neurons in the substantia nigra pars compacta (SNC), (arrows). SNR=substantia nigra pars reticulata. Cell nuclei are stained with DAPI (blue).
- (C)NGTDPYKPK(S)PESD, corresponding to amino acid residues 999 - 1012 of human TRPM2 (Accession O94759).