Overview
Type: Synthetic peptide
Form: Lyophilized powder
TRPM2 Blocking Peptide (#BLP-CC043) is the original antigen used for immunization during Anti-TRPM2 Antibody (#ACC-043) generation. The blocking peptide binds and ‘blocks’ Anti-TRPM2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat lung membrane (lanes 1 and 4), rat brain membrane (lanes 2 and 5) and human K562 chronic myelogeneous cells (lanes 3 and 6) lysates:1-3. Anti-TRPM2 Antibody (#ACC-043), (1:200).
4-6. Anti-TRPM2 Antibody, preincubated with TRPM2 Blocking Peptide (#BLP-CC043).
Expression of TRPM2 in rat lungImmunohistochemical staining of rat lung paraffin embedded sections using Anti-TRPM2 Antibody (#ACC-043), (1:100). TRPM2 is expressed in the respiratory epithelium (black arrows). In addition, staining is also present in smooth muscle, both bronchiolar (arrowheads) and vascular (red arrows). Hematoxilin is used as the counterstain.
Properties
Sequence
- (C)HTFQGKEWDPKKHVQE, corresponding to amino acid residues 105-120 of mouse TRPM2 (Accession Q91YD4).
Accession (Uniprot) Number Q91YD4
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
