TRPM2 Blocking Peptide (#BLP-CC043) is the original antigen used for immunization during Anti-TRPM2 Antibody (#ACC-043) generation. The blocking peptide binds and ‘blocks’ Anti-TRPM2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat lung membrane (lanes 1 and 4), rat brain membrane (lanes 2 and 5) and human K562 chronic myelogeneous cells (lanes 3 and 6) lysates:
- Expression of TRPM2 in rat lungImmunohistochemical staining of rat lung paraffin embedded sections using Anti-TRPM2 Antibody (#ACC-043), (1:100). TRPM2 is expressed in the respiratory epithelium (black arrows). In addition, staining is also present in smooth muscle, both bronchiolar (arrowheads) and vascular (red arrows). Hematoxilin is used as the counterstain.
- (C)HTFQGKEWDPKKHVQE, corresponding to amino acid residues 105-120 of mouse TRPM2 (Accession Q91YD4).