TRPML2/Mucolipin-2 Blocking Peptide (#BLP-CC082) is the original antigen used for immunization during Anti-TRPML2 (Mucolipin-2) Antibody (#ACC-082) generation. The blocking peptide binds and ‘blocks’ Anti-TRPML2/Mucolipin-2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of human MCF-7 breast adenocarcinoma cell line (lanes 1 and 4), rat heart (lanes 2 and 5) and mouse heart (lanes 3 and 6) lysates:
- Expression of TRPML2 in rat heartImmunohistochemical staining of rat heart paraffin-embedded sections using Anti-TRPML2 (Mucolipin-2) Antibody (#ACC-082), followed by AlexaFluor-555 secondary antibody. A. TRPML2 labeling appears specifically in the cardiac muscle fibers and in a lesser intensity in the endothelium. It does not appear in the smooth muscle fibers of blood vessels. B. Nuclear staining using DAPI as the counterstain. C. Merged images of A and B.
- (C)RLDFYRLVQVDISFALK, corresponding to amino acid residues 212-228 of mouse TRPML2 (Accession Q8K595).