Overview
Cat #: BLP-GC036
Type: Synthetic peptide
Form: Lyophilized powder
A Blocking peptide for Anti-VGLUT2 Antibody (#AGC-036), Anti-VGLUT2-ATTO Fluor-594 Antibody (#AGC-036-AR) and Guinea Pig Anti-VGLUT2 Antibody (#AGP-131). Intended to be used as a negative control.
Applications: bl
Application key:
BL- Blocking
For research purposes only, not for human use
Applications
Applications
Blocking
- Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell lysate (lanes 3 and 6):1-3. Anti-VGLUT2 Antibody (#AGC-036), (1:400).
4-6. Anti-VGLUT2 Antibody, preincubated with VGLUT2 Blocking Peptide (#BLP-GC036). - Expression of VGLUT2 in rat neocortexImmunohistochemical staining of rat frozen brain sections using Anti-VGLUT2 Antibody (#AGC-036), (1:400). VGLUT2 (red) is detected in pyramidal neurons (arrow). The same section was stained with DAPI (blue).
- Multiplex staining of VGLUT2 and P2X7 Receptor in rat spinal cordImmunohistochemical staining of perfusion-fixed frozen rat spinal cord sections using Anti-VGLUT2 Antibody (#AGC-036), (1:600) and Anti-P2X7 Receptor-ATTO Fluor-550 Antibody (#APR-004-AO), (1:100). A. Vesicular glutamate transporter 2 labeling followed by goat-anti-rabbit-Alexa-488 (green). B. The same section labeled for P2X7 receptor (orange). C. Merge of A and B demonstrates partial co-localization of VGLUT2 and P2X7 receptor in dorsal horn and in lateral column (L. Col., arrow). Cell nuclei were stained with DAPI (blue).
- Multiplex staining of VGLUT2 and Neurokinin 1 Receptor in rat DRGImmunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglion (DRG) sections using Anti-VGLUT2-ATTO Fluor-594 Antibody (#AGC-036-AR), (1:60) and Anti-Neurokinin 1 Receptor (NK1R) (extracellular)-ATTO Fluor-488 Antibody (#ATR-001-AG), (1:60). A. VGLUT2 staining (red). B. NK1 receptor staining (green). C. Merge of the two images demonstrates co-localization in some neuronal bodies (arrows point at examples). Cell nuclei are stained with DAPI (blue).
- Multiplex staining of AT2 Receptor and VGLUT2 in rat supraoptic hypothalamic nucleusImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO Fluor-488 Antibody (#AAR-012-AG), (1:60) and Anti-VGLUT2-ATTO Fluor-594 Antibody (#AGC-036-AR), (1:60). A. AT2 receptor staining (green). B. VGLUT2 staining (red). C. Merge of the two images shows a general lack of co-localization of AT2 and VGLUT2 in this part of the hypothalamus (arrow). Cell nuclei are stained with DAPI (blue).
- Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Guinea Pig Anti-VGLUT2 Antibody (#AGP-131), (1:200). 3-4. Guinea Pig Anti-VGLUT2 Antibody, preincubated with VGLUT2 Blocking Peptide (BLP-GC036).
- Expression of VGLUT2 in rat parietal cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-VGLUT2 Antibody (#AGP-131), (1:200), followed by goat anti-Guinea Pig-AlexaFluor-594. A. VGLUT2 immunoreactivity (red) appears in pyramidal neuronal profiles (horizontal arrows). B. Pre-incubation of the antibody with VGLUT2 Blocking Peptide (BLP-GC036), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Properties
Sequence
Accession (Uniprot) Number Q9JI12
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
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