Name: WS 12
Brand: Alomone Labs
SKU: W-105

Cat #: W-105

Alternative Name (1R*,2S*)-N-(4-Methoxyphenyl)-5-methyl-2-(1-methylethyl)cyclohexanecarboxamide

Lyophilized Powder yes

Bioassay Tested

Source Synthetic

MW: 289.41

Purity: >99%

Effective concentration 1 nM – 10 µM.

Structure

Chemical name (1R,2S,5R)-N-(4-methoxyphenyl)-5-methyl-2-propan-2-ylcyclohexane-1-carboxamide.

Molecular formula C₁₈H₂₇NO₂.

CAS No.: 68489-09-8

PubChem CID 11266244

Activity WS 12 is a TRPM8 channel activator (EC₅₀= 193 nM)¹.

References-Activity

Bodding, M. et al. (2007) Cell Calcium 42, 618.

Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Solubility Up to 75 mM in Ethanol. Centrifuge all products before use (10000 x g 5 min).

Storage of solutions Up to three months at -20°C.

Our bioassay

Alomone Labs WS 12 activates TRPM8 channels expressed in HEK-293 cells.
Dose response curve for the activation of human TRPM8 by rising concentrations of WS 12 (#W-105), showing activity at low nanomolar range. Cells were loaded with Calcium-5 dye. Changes in intracellular Ca²⁺were detected as changes in 485 nm/525 nm fluorescence following agonist application.

Scientific background

The carboxamide WS 12 is a synthetic selective TRPM8 channel agonist¹.

TRPM8 is a Ca²⁺-permeable channel, a member of the ‘long' or melastatin (M) group of TRP proteins and is activated by temperatures or by cooling compounds such as icilin, menthol and eucalyptol. The TRPM8 gene is expressed and is up-regulated in prostate cancer and other malignancies.It is specifically expressed in temperature-sensing trigeminal and dorsal root ganglion neurons^1-3.

Target TRPM8 channel

Lyophilized Powder

Last update: 06/11/2022

For research purposes only, not for human use

Specifications

Citations

Applications

Scientific Background

Shipping and Ordering information

Order Summary

Certificate of Analysis

WS 12

Specifications

Citations

Applications

Scientific Background

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