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3,4,6. Anti-TRPM8 (extracellular) Antibody, preincubated with the control peptide antigen.
- Fleig, A. et al. (2004) Trends. Pharmacol. Sci. 25, 633.
- Brauchi, S. et al. (2004) Proc. Natl. Acad. Sci. 101, 15494.
- McKemy, D.D. et al. (2002) Nature 416, 52.
- Peier, A.M. et al. (2002) Cell 108, 705.
- Dragoni, I. et al. (2006) J. Biol. Chem. 281, 37353.
- Zhang, L. et al. (2006) Endocr. Relat. Cancer 13, 27.
The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first member, melastatin (TRPM1), whose gene was identified in metastatic and benign melanomas.1 The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N-terminus and C-terminus. However, due to their long N-terminus and C-terminus they were also named the long TRP channel family.1
The TRPM8 channel is the cold and menthol receptor activated by either cold temperatures(~28°C) or menthol.2
TRPM8 is expressed in dorsal root ganglia (DRGs) where about 5%-10% of the small diameter DRG neurons express the channel.3,4 In DRGs, TRPM8 expressing neurons do not express the TRPV1 channel.5 Overexpression of TRPM8 was found in prostate cancer cells. However, the physiological and pathological roles that these cells play is still elusive.6 In prostate, it was suggested that TRPM8 might play a possible role in the progression of cancer to the metastatic stage.6
Species reactivity key:
Expression of TRPM8 in rat tail artery astrocytes.Immunocytochemical staining of rat tail artery astrocytes using Anti-TRPM8 (extracellular) Antibody (#ACC-049). TRPM8 staining is shown in green, nuclei are in blue. Omission of the antibody (lower panel) abolishes TRPM8 staining.Adapted from Johnson, C.D. et al. (2009) Am. J. Physiol. 296, H1868. with permission of the American Physiological Society.
Anti-TRPM8 (extracellular) Antibody (#ACC-049) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, immunocytochemistry, and neutralization applications. It has been designed to recognize TRPM8 from human, rat, and mouse samples.
Adapted from Miller, S. et al. (2014) with kind permission of Dr. Gavva, N.R. Department of Neuroscience, Amgen Inc., California, U.S.A.
- Rat DRG sections (1:300).
Vinuela-Fernandez, I. et al. (2014) Neuropharmocolgy 79, 136.
- Rat tail artery VSMC (1:200).
Melanaphy, D. et al. (2016) Am. J. Physiol. 311, H1416.
- Rat F11 dorsal root ganglion hybridoma cells (1:400).
Toro, C.A. et al. (2015) J. Neurosci. 35, 571.
- Human prostate cancer DU145 cells (1:50).
Wang, Y. et al. (2012) Pathol. Oncol. Res. 18, 903.
- Rat tail artery astrocytes.
Johnson, C.D. et al. (2009) Am. J. Physiol. 296, H1868.
- Human, mouse and rat TRPM8 expressed in CHO cells, and rat DRGs.
Miller, S. et al. (2014) PLoS ONE 9, e107151.