ZnT10 (SLC30A10) Blocking Peptide (#BLP-ZT020) is the original antigen used for immunization during Anti-ZnT10 (SLC30A10) Antibody (#AZT-020) generation. The blocking peptide binds and ‘blocks’ Anti-ZnT10 (SLC30A10) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat small intestine lysate (lanes 1 and 5), mouse liver lysate (lanes 2 and 6), rat liver lysate (lanes 3 and 7) and rat brain lysate (lanes 4 and 8):
- Western blot analysis of human SHSY-5Y neuroblastoma cell line lysate:
- Expression of ZnT10 in rat basal ganglia.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-ZnT10 (SLC30A10) Antibody (#AZT-020), (1:1200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat substantia nigra region, shows ZnT10 immunoreactivity (green) in neuronal profiles (arrows). B. Pre-incubation of the antibody with ZnT10 (SLC30A10) Blocking Peptide (BLP-ZT020), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Peptide (C)DASTKIREIFHHAG, corresponding to amino acid residues 361 - 374 of human ZnT10 (Accession Q6XR72).