This product is freeze dried. All water molecules have been removed.
Every lot is tried & tested in a relevant biological assay.
Alomone Labs α-Bungarotoxin-ATTO-633 inhibits muscle nACh receptors heterologously expressed in Xenopus oocytes.A. Time course of muscle nicotinic ACh channels current recording. Membrane potential was held at -80 mV and stimulated every 100 sec with a solution containing 100 µM ACh + 3 μM PNU-120596. 50 nM α-Bungarotoxin-ATTO-633 (#B-100-FR) was applied (green) for 5 min during the ACh application and inhibited channel current. B. Superimposed examples of muscle nicotinic ACh current in the absence (black) and presence (green) of 50 nM α-Bungarotoxin-ATTO-633 (taken from the experiment in A).
α-Bungarotoxin isoform A31 is a 74 amino acid peptidyl toxin isolated from the venom of the banded krait snake, Bungarus multicinctus1.
α-Bungarotoxin blocks postsynaptic neuromuscular transmission via competitive inhibition of nicotinic ACh receptors (nAChRs) with an IC50 of 3.5 x 10-10 M, thereby prevents the depolarizing action on postsynaptic membranes and blocks neuromuscular transmission2.
The toxin is selective for α7 receptors (IC50 value of 1.6 nM) and α3/β4 receptors (IC50 value of > 3 µM)3,4.
α-Bungarotoxin also binds to and blocks a subset of GABAA receptors (GABAARs) that contain the GABAAR β3 subunit. In particular, α-Bungarotoxin blocks GABAARs that contain interfaces between adjacent β3 subunits5.
Alomone Labs α-Bungarotoxin-ATTO-633 in whole mount staining of mice neuromuscular junction (NMJ)Whole mount staining of mouse neuromuscular junction (NMJ) was stained with the NMJ marker α-Bungarotoxin-ATTO-633 (#B-100-FR) (purple) at 1 µg/ml concentration.The image was taken using Nikon Epifluorescence microscopy at 60X magnification and is kindly provided by Dr. Eran Perlsson, Dept. of Physiology and Pharmacology, Tel-Aviv University, Tel-Aviv, Israel.
α-Bungarotoxin-ATTO-633 (#B-100-FR) is a highly pure, natural, and biologically active conjugated peptide toxin.
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- α-Bungarotoxin (#B-100)
- α-Bungarotoxin-ATTO-488 (#B-100-AG)
- α-Conotoxin LtIA (#STC-550)
- α-Conotoxin AuIB (#STC-310)
- α-Conotoxin ImI (#C-290)
- Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody (#ANC-007)
- Anti-Nicotinic Acetylcholine Receptor α1 (CHRNA1) (extracellular) Antibody (#ANC-001)
- α-Bungarotoxin-Biotin (#B-100-B)
- α-Bungarotoxin-FITC (#B-100-F)