Name: ω-Conotoxin CnVIIA
Brand: Alomone Labs
SKU: STC-800

Cat #: STC-800

Lyophilized Powder yes

Bioassay Tested

Origin Synthetic peptide

MW: 2848 Da

Purity: >98% (HPLC)

Effective concentration 1-5 µM.

Sequence CKGKGAXCTRLMYDCCHGSCSSSKGRC.

Modifications Disulfide bonds between Cys¹-Cys¹⁶, Cys⁸-Cys²⁰and Cys¹⁵-Cys²⁷. X = Hydroxyproline. Cys²⁷ - C-terminal amidation.

Structure

Molecular formula C₁₁₀H₁₇₉N₃₉O₃₆S₇.

Activity ω-Conotoxin CNVIIA inhibits N-type Ca²⁺ channels¹.

References-Activity

Favreau, P. et al. (2001) Biochemistry 40, 14567.

Accession number P58916.

Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Solubility Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions Up to two weeks at 4°C or three months at -20°C.

Our bioassay

Alomone Labs ω-Conotoxin CnVIIA inhibits Ca_V2.2 channels heterologously expressed in Xenopus oocytes.
A. Time course of ω-Conotoxin CnVIIA (#STC-800) action on Ca_V2.2 + α2δ1 + β1 currents. Peak current amplitude was plotted as a function of time. Membrane potential was held at -100 mV and oocytes were stimulated by a 100 ms voltage ramp to +50 mV. 1 µM ω-Conotoxin CnVIIA (applied for 180 sec, green) was perfused during the period marked by the bar, as indicated and showed 75% inhibition of control current. B. Superimposed traces of channel current in the absence (black) and presence (green) of 1 µM ω-Conotoxin CnVIIA (taken from experiment in A).

References - Scientific background

Qin, N. et al. (2002) Mol. Pharmacol. 62, 485.
Favreau, P. et al. (2001) Biochemistry 40, 14567.
Lewis R.J. et al. (2012) Pharmacol. Rev. 64, 259.

Scientific background

Ca²⁺ channels have been classified pharmacologically and biophysically. The channels are designated L-, T-, N-, P-, Q- and R-type. Each channel has different voltage ranges and rates for activation and inactivation. The N-type channels are typically inactivated more rapidly than the L-, P- and Q-types¹.

ω-Conotoxin CNVIIA is a specific voltage-sensitive calcium channel blocker. It was first isolated from the fish-hunting cone snail Conus consors venom. This peptide consists of 27 amino acid residues folded by 3 disulfide bridges². ω-Conotoxin CNVIIA exhibits a clear selectivity for N-type specific voltage-sensitive Ca²⁺ channels². The therapeutic potential of ω-conotoxins is based on their ability to selectively inhibit mammalian Ca_V isoforms expressed in ascending pain pathways (especially Ca_V2.2, which underlies the N-type current present in sensory neurons) and to produce antinociception in animal models of pain³.

Target N-type Ca²⁺channels

Peptide Content: 100%

Lyophilized Powder

Last update: 02/01/2022

ω-Conotoxin CnVIIA (#STC-800) is a highly pure, synthetic, and biologically active peptide toxin.

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ω-Conotoxin CnVIIA

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