AKAP150 Blocking Peptide (#BLP-PZ031) is the original antigen used for immunization during Anti-AKAP150 Antibody (#APZ-031) generation. The blocking peptide binds and ‘blocks’ Anti-AKAP150 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), rat dorsal root ganglion lysates (lanes 3 and 7) and mouse lung lysates (lanes 4 and 8):
- (C)RGAKRSRPSKLTED, corresponding to amino acid residues 128-141 of rat AKAP5 (Accession P24587).