Anti-AKAP150 Antibody

A-kinase anchor protein 150kD, A-kinase anchor protein 5, AKAP5
    Cat #: APZ-031
    Alternative Name A-kinase anchor protein 150kD, A-kinase anchor protein 5, AKAP5
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
      • Peptide (C)RGAKRSRPSKLTED, corresponding to amino acid residues 128-141 of rat AKAP5 (Accession P24587). Intracellular.
        Anti-AKAP150 Antibody
    Accession (Uniprot) Number P24587
    Gene ID 171026
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – 13/14 amino acid residues identical.
    RRID AB_2756780.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity The antibody won’t recognize the human AKAP150 homologue (also known as AKAP79).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
      • Anti-AKAP150 Antibody
        Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), rat dorsal root ganglion lysates (lanes 3 and 7) and mouse lung lysates (lanes 4 and 8):
        1-4. Anti-AKAP150 Antibody (#APZ-031), (1:200).
        5-8. Anti-AKAP150 Antibody, preincubated with the negative control antigen.
    References
      • A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins that bind to the regulatory unit of Protein Kinase A (PKA) to discrete locations within the cell1. AKAPs include two conserved structural modules: a targeting domain that serves as a scaffold and membrane anchor that consists of two NH2-terminal structural modules that are highly conserved among mammalian homologues. They also have a tethering domain that interacts with PKA regulatory subunits which consists of a short amphipathic helix. Alternative splicing can shuffle targeting and tethering domains to generate a variety of AKAPs with different targeting specificity. Although AKAPs have been identified on the basis of their interaction with PKA, they also bind other signaling molecules, mainly phosphatases and kinases that regulate AKAP targeting and activate other signal transduction pathways2.

        Some types of AKAPs interact with CaV1.1 and CaV1.2. These voltage-gated calcium channels are phosphorylated by PKA after β-adrenergic activation. This phosphorylation is enabled by the binding of AKAP150 to the distal C-terminal of CaV1.1 and CaV1.2. Binding of different AKAPs that interact with distinct signaling partners to the calcium channel intracellular C-terminal domain may act to fine-tune channel activity and downstream calcium-mediated processes3.

        A single subtype of neuronal AKAP is expressed widely in the brain and is highly conserved in mouse (150 kDA), bovine (75 kDA) and human (79 kDA). APAK150 targets PKAII to the postsynaptic cytoskeleton and perikarya of neurons. It is expressed in Purkinje cells and in olfactory bulb neurons, basal ganglia, cerebral cortex and other forebrain areas. AKAP150 is concentrated in primary branches of dendrites in association with microtubules. The localization of PKAIIβ in brain overlaps with that of AKAP150, suggesting that AKAP150 is the relevant PKAIIβ anchor protein in the brain2.

        Inhibition of either AKAP150 or TRPV1 binding sites causes an abolishment of PKC-dependent TRPV1 sensitization to capsaicin stimuli thus making the AKAP150-TRPV1 interaction a viable target for novel analgesics4.

        AKAP150 interacts with another group of scaffolding proteins known as the SAP90/PSD95-associated protein family. AKAP150 interacts directly with the SH3 and GK domains of PSD95 and SAP97. These proteins have specific roles in synaptic localization of NMDARs and AMPARs respectively5.

        Post myocardial infarction, AKAP150-mediated targeting of calcineurin to sarcolemmal micro-domains in ventricular myocytes contributes to the local and acute gene remodeling events that lead to the down-regulation of KV currents6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 15/08/2019

    Anti-AKAP150 Antibody (#APZ-031) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize AKAP150 from mouse and rat samples. The antibody is unlikely to recognize AKAP5 from human samples.

    For research purposes only, not for human use