Overview
Type: Synthetic peptide
Form: Lyophilized powder
Alpha 1 Na+/K+ ATPase Blocking Peptide (#BLP-NP001) is the original antigen used for immunization during Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001) generation. The blocking peptide binds and ‘blocks’ Anti-Alpha 1 Na+/K+ ATPase primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001), (1:200).
3,4. Anti-Alpha 1 Na+/K+ ATPase Antibody, preincubated with Alpha 1 Na+/K+ ATPase Blocking Peptide (#BLP-NP001).
Western blot analysis of human Colo-205 colon adenocarcinoma cell lysate:1. Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001), (1:200).
2. Anti-Alpha 1 Na+/K+ ATPase Antibody, preincubated with Alpha 1 Na+/K+ ATPase Blocking Peptide (#BLP-NP001).
Note: Samples should be heated up to 37°C. Do not boil samples.
Expression of Na+/K+ ATPase α1 (ATP1A1) in rat kidneyImmunohistochemical staining of paraffin embedded rat kidney sections using Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001), (1:200). ATP1A1 staining (brown) is present in collecting ducts and thick portions of the Henle loop in the renal medulla. Hematoxilin is used as the counterstain.
Expression of Na+/K+ ATPase α1 (ATP1A1) in mouse brainImmunohistochemical staining of immersion-fixed, free floating mouse brain frozen sections using Anti-Alpha 1 Na+/K+ ATPase Antibody (#ANP-001), (1:400). ATP1A1 staining (red) is apparent in the hippocampal dentate gyrus in neuronal soma (vertical arrows) and in astrocytes (horizontal arrow). DAPI (blue) is used to visualize cell nuclei.
Properties
Sequence
- (C)RDKYEPAAVSEHGD, corresponding to amino acid residues 7- 20 of rat ATP1A1 (Accession P06685).
Accession (Uniprot) Number P06685
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
