Anoctamin-6 (extracellular) Blocking Peptide (#BLP-CL016) is the original antigen used for immunization during Anti-Anoctamin-6 (extracellular) Antibody (#ACL-016) generation. The blocking peptide binds and ‘blocks’ Anti-Anoctamin-6 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse liver lysate (lanes 1 and 3) and rat liver membranes (lanes 2 and 4):
- Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human MEG-01 megakaryoblastic leukemia cell line lysate (lanes 2 and 4):
- (C)KLPKNINGTDPIQK, corresponding to amino acid residues 487-500 of human ANO6 (Accession Q4KMQ2).