Overview
Type: Synthetic peptide
Form: Lyophilized powder
Anoctamin-6 (extracellular) Blocking Peptide (#BLP-CL016) is the original antigen used for immunization during Anti-Anoctamin-6 (extracellular) Antibody (#ACL-016) generation. The blocking peptide binds and ‘blocks’ Anti-Anoctamin-6 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse liver lysate (lanes 1 and 3) and rat liver membranes (lanes 2 and 4):1,2. Anti-Anoctamin-6 (extracellular) Antibody (#ACL-016), (1:200).
3,4. Anti-Anoctamin-6 (extracellular) Antibody, preincubated with Anoctamin-6 (extracellular) Blocking Peptide (#BLP-CL016).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human MEG-01 megakaryoblastic leukemia cell line lysate (lanes 2 and 4):1,2. Anti-Anoctamin-6 (extracellular) Antibody (#ACL-016), (1:200).
3,4. Anti-Anoctamin-6 (extracellular) Antibody, preincubated with Anoctamin-6 (extracellular) Blocking Peptide (#BLP-CL016).
Properties
Sequence
- (C)KLPKNINGTDPIQK, corresponding to amino acid residues 487-500 of human ANO6 (Accession Q4KMQ2).
Accession (Uniprot) Number Q4KMQ2
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
