Anti-Angiotensin-(1-7) Mas Receptor Antibody

Proto-oncogene Mas, MAS1
    Cat #: AAR-013
    Alternative Name Proto-oncogene Mas, MAS1
  • KO Validated
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)KIRKNTWASHSSK, corresponding to amino acid residues 212-224 of the rat Angiotensin-(1-7) Mas receptor (Accession P12526). 3rd intracellular loop.
    • Anti-Angiotensin-(1-7) Mas Receptor Antibody
    Accession (Uniprot) Number P12526
    Gene ID 25153
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human - identical.
    RRID AB_2039972.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
    Western blot
    • Anti-Angiotensin-(1-7) Mas Receptor Antibody
      Western blot analysis of rat brain (lanes 1 and 4) kidney (lanes 2 and 5) and heart (lanes 3 and 6) membranes:
      1-3. Anti-Angiotensin-(1-7) Mas Receptor Antibody (AAR-013), (1:200).
      4-6. Anti-Angiotensin-(1-7) Mas Receptor Antibody, preincubated with the control peptide antigen.
    • Anti-Angiotensin-(1-7) Mas Receptor Antibody
      Western blot analysis of mouse kidney membranes:
      1. Anti-Angiotensin-(1-7) Mas Receptor Antibody (AAR-013), (1:200).
      2. Anti-Angiotensin-(1-7) Mas Receptor Antibody, preincubated with the control peptide antigen.
    • Anti-Angiotensin-(1-7) Mas Receptor Antibody
      Western blot analysis of human HeLa cervix adenocarcinoma cell line lysate:
      1. Anti-Angiotensin-(1-7) Mas Receptor Antibody (AAR-013),(1:200).
      2. Anti-Angiotensin-(1-7) Mas Receptor Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    • Anti-Angiotensin-(1-7) Mas Receptor Antibody
      Expression of Angiotensin-(1-7) Mas Receptor in rat kidney
      Immunohistochemical staining of rat kidney paraffin embedded sections using Anti-Angiotensin-(1-7) Mas Receptor Antibody (#AAR-013), (1:100). Angiotensin-(1-7) Mas receptor (brown staining) is detected in proximal tubules (PT) and distal tubules (DT) in the renal cortex. Collecting ducts (CD) are less stained and both glomeruli (G) and blood vessels (A) are negative.  Hematoxilin is used as the counterstain.
    Immunocytochemistry
    • Mouse microglia culture (1:20) (Foulquier, S. et al. (2019) Angiogenesis 22, 481.).
    References
    1. Santos, R.A. et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 8258.
    2. Alenina, N. et al. (2008) Exp. Physiol. 93, 528.
    3. Santos, R.A. et al. (2008) Exp. Physiol. 93, 519.
    Scientific background

    The Angiotensin-(1-7) Mas receptor is the recently identified receptor of the biologically active heptapeptide Angiotensin-(1-7).1

    Angiotensin (Ang)-(1-7) is a metabolite of the well known peptide hormone Angiotensin (Ang) II, a key component of the renin-angiotensin system (RAS) that has a central role in cardiovascular homeostasis.

    Considerably interest in Ang-(1-7) and its receptor aroused in the last few years since it became apparent that it can counterbalance most of Ang II effects. Thus Ang-(1-7) has vasodilator and hypotensive effects as well as antiarrhythmic and cardioprotective roles.2, 3

    The Ang-(1-7) Mas receptor belongs to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily and was originally described as a protooncogene.

    Signaling via the Ang-(1-7) Mas receptor is still poorly elucidated however, evidence indicates that the receptor is coupled to a Gq/11 protein that activates phospholipase C (PLC).2,3

    The Ang-(1-7) Mas receptor is expressed in several organs including heart, kidney, blood vessels, testis and brain. Studies with Ang-(1-7) Mas receptor knockout mice have demonstrated the key role of this receptor in cardiovascular regulation as well as in the regulation of learning and memory.2

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-Angiotensin-(1-7) Mas Receptor Antibody
    Expression of Angiotensin-(1-7) Mas Receptor in sheep kidney.Immunohistochemical staining of sheep kidney sections using Anti-Angiotensin-(1-7) Mas Receptor Antibody (#AAR-013). A. Mas receptor staining in the cortex (green) is mostly associated with the proximal tubules and is absent in in the glomerulus. B. Mas receptor staining is observed in the collecting ducts in the medullary region. C. The limb of Henle also shows Mas receptor staining. D-F. Mas receptor staining is abolished when the antibody is pre-incubated with the control peptide antigen.Adapted from Gwathmey, T.M. et al. (2010) Am. J. Physiol. 299, F983. with permission of The American Physiological Society.
    Last update: 03/06/2020

    Anti-Angiotensin-(1-7) Mas Receptor Antibody (#AAR-013) is a highly specific antibody designed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize Ang-(1-7) Mas receptor from rat, mouse, and human samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Published figures using this product
    • Anti-Angiotensin-(1-7) Mas Receptor Antibody
      Expression of Angiotensin-(1-7) Mas Receptor in mouse heart and kidney.
      A, C. Immunohistochemical staining of mouse over-expressing growth hormone (GH) using Anti-Angiotensin-(1-7) Mas Receptor Antibody (#AAR-013). Mas receptor staining decreases in mice over-expressing GH (right panels). B, D. Western blot analysis of same tissues in A and C using Anti-Angiotensin-(1-7) Mas Receptor Antibody also shows a decrease in Mas receptor’s expression.
      Adapted from Munoz, M.C. et al. (2014) with permission of the Society for Endocrinology.
    KO validation citations
    1. Mouse brain sections. Tested in MAS-/- mice.
      Freund, T. et al. (2012) Cell Tissue Res. 348, 29.
    Western blot citations
    1. Mouse brain and macrophage lysate (1:250).
      Hammer, A. et al. (2016) Proc. Natl. Acad. Sci. U.S.A. 113, 14109.
    2. Mouse brain lysate (1:2000).
      Uekawa, K. et al. (2016) J. Alzheimers Dis. 53, 127.
    3. Mouse kidney lysate.
      Ali, Q. et al. (2016) J. Renin Angiotensin Aldosterone Syst. 17, 3.
    4. Sheep kidney lysate (1:250).
      Wilson, B.A. et al. (2016) Am. J. Physiol. 310, F637.
    5. Rat carotid arteries lysate (1:500).
      Olivon, V.C. et al. (2015) Peptides 71, 250.
    6. Rat heart lysates.
      Hao, P.P. et al. (2015) Am. J. Physiol. 308, H1007.
    7. Rat kidney lysate.
      Zimmerman, M.A. et al. (2014) Am. J. Physiol. 306, F1136.
    8. Mouse kidney lysate (1:4000).
      Munoz, M.C. et al. (2014) J. Endocrinol. 221, 215.
    9. Mouse skeletal muscle lysate (1:7500).
      Sabharawal, R. et al. (2014) Clin. Sci. 127, 101.
    10. Rat brain lysate (1:2000).
      Du, D. et al. (2013) PLoS ONE 8, e70976.
    11. Mouse penis lysate (1:200).
      Fraga Silva, R.A. et al. (2013) J. Sex. Med. 10, 2430.
    12. Rat heart lysate (1:5000).
      Wang, H. et al. (2013) PLoS ONE 8, 76992.
    13. Mouse lung lysate (1:500).
      Rodrigues-Machado, M.G. et al. (2013) Br. J. Pharmacol. 170, 835.
    14. Sheep brain medulla lysate (1:250).
      Marshall, A.C. et al. (2013) Peptides 44, 25.
    Immunohistochemistry citations
    1. Rat carotid sections (1:300).
      Olivon, V.C. et al. (2015) Peptides 71, 250.
    2. Mouse uterine artery and placenta sections (1:400).
      Yamaleyeva, L.M. et al. (2015) Am. J. Physiol. 309, E84.
    3. Mouse kidney sections (1:100).
      Munoz, M.C. et al. (2014) J. Endocrinol. 221, 215.
    4. Mouse skeletal muscle sections (1:200).
      Sabharawal, R. et al. (2014) Clin. Sci. 127, 101.
    5. Rat brain sections.
      Freund, M. et al. (2014) Eur. Neuropsychopharmacol. 24, 302.
    6. Rat striatum.
      Regenhardt, R.W. et al. (2014) Exp. Physiol. 99, 442.
    7. Mouse brain sections. Tested in MAS-/- mice.
      Freund, T. et al. (2012) Cell Tissue Res. 348, 29.
    8. Sheep kidney sections.
      Gwathmey, T.M. et al. (2010) Am. J. Physiol. 299, F983.
    Immunocytochemistry citations
    1. Mouse microglia culture (1:20).
      Foulquier, S. et al. (2019) Angiogenesis 22, 481.
    Immunofluorescence citations
    1. Mouse microglia culture (1:20).
      Foulquier, S. et al. (2019) Angiogenesis 22, 481.
    2. Mouse brain sections. Tested in MAS-/- mice.
      Freund, T. et al. (2012) Cell Tissue Res. 348, 29.
    More product citations
    1. Freund, M. et al. (2012) Cell Tissue Res. 348, 29.
    2. Lakshmanan, A.P. et al. (2012) Biochem. Pharmacol. 83, 653.
    3. Muñoz, M.C. et al. (2012) Regul. Pept. 177, 1.
    4. Oh, Y.B. et al. (2012) Peptides. 37, 79.
    5. Sukumaran, V. et al. (2012) Life Sci. 90, 289.
    6. Silva, D.M. et al. (2011) J. Appl. Physiol. 111, 1272.
    7. Sukumaran, V. et al. (2011) Int. J. Biol. Sci. 7, 1077.
    8. Yang, R.F. et al. (2011) Am. J. Physiol. 300, C58.
    9. Ferreira, A.J. et al. (2010) Ther. Adv. Cardiovasc. Dis. 4, 83.
    10. Gwathmey, T.M. et al. (2010) Hypertension 55, 166.
    11. Varagic, J. et al. (2010) Am. J. Nephrol. 32, 557.
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