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Guinea pig Anti-ASIC3 Antibody

Acid-sensing ion channel 3, Amiloride-sensitive cation channel 3, ACCN3, DRASIC

Cat #: AGP-052
Alternative Name Acid-sensing ion channel 3, Amiloride-sensitive cation channel 3, ACCN3, DRASIC
Lyophilized Powder yes
Type: Polyclonal
Host: Guinea pig
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide KPRSGLEEAQRRQAS(C), corresponding to amino acid residues 2-16 of rat ASIC3 (Accession O35240). Intracellular, N-terminus.
Accession (Uniprot) Number O35240
Gene ID 286920
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 14/15 amino acid residues identical; human - 11/15 amino acid residues identical.
RRID AB_2756612.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Guinea pig total IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    1,2. Guinea pig Anti-ASIC3 Antibody (#AGP-052), (1:1000).
    3,4. Guinea pig Anti-ASIC3 Antibody, preincubated with ASIC3 Blocking Peptide (#BLP-SC018).
  • Western blot analysis of rat dorsal root ganglion (DRG) lysate:
    Western blot analysis of rat dorsal root ganglion (DRG) lysate:
    1. Guinea pig Anti-ASIC3 Antibody (#AGP-052), (1:1000).
    2. Guinea pig Anti-ASIC3 Antibody, preincubated with ASIC3 Blocking Peptide (#BLP-SC018).
Immunohistochemistry
  • Expression of ASIC3 in mouse dorsal raphe nucleus
    Expression of ASIC3 in mouse dorsal raphe nucleus
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Guinea pig Anti-ASIC3 Antibody (#AGP-052), (1:200), followed by goat-anti-guinea pig-AlexaFluor-488 secondary antibody. ASIC3 staining (green) appears in the soma of dorsal raphe nucleus (DRN) neurons (arrows). Nuclei are stained with DAPI (blue).
References
  1. Kellenberger, S. and Schild, L. (2002) Physiol. Rev. 82, 735.
  2. Chen, C.C. et al. (2002) Proc. Natl. Acad. Sci. U.S.A. 99, 8992.
  3. Price, M.P. et al. (2001) Neuron 32, 1071.
Scientific background

ASIC3 is a member of a family of Na+ channels that are activated by external protons. The family includes four additional members: ASIC1ASIC2ASIC4 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin/epithelial Na+ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini.

The functional channel is composed of 4 subunits that can be assembled as homo- or heterotetramers with the other ASIC subunits.

A drop in external pH opens the channel resulting in an inward cation current that is quickly inactivated even in the continuous presence of protons in the medium, although a small residual current may persist.

Several lines of evidence indicate that ASIC3 may function as a pain sensor. First, it is specifically located in dorsal root ganglia (DRG) neurons and in nociceptors that are involved in pain detection and transmission. In addition, in conditions such as inflammation and cardiac ischemia that include tissue acidosis and thus pain, ASIC3 currents have been detected. Finally, ASIC3 deficient mice display altered sensitivity to high intensity pain produced by heat or acid.

Interestingly, while in rats ASIC3 is expressed almost exclusively in DRG neurons, in humans its expression is more widespread suggesting a more extensive role in human nociception.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat ASIC3. Guinea pig Anti-ASIC3 Antibody (#AGP-052) raised in guinea pig can be used in western blot and immunohistochemistry applications. It has been designed to recognize ASIC3 from rat, human, and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-ASIC3 Antibody (#ASC-018) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

For research purposes only, not for human use
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