Anti-ASIC5 (extracellular) Antibody

Acid-sensing ion channel 5, Amiloride-sensitive cation channel 5, Brain-liver-intestine amiloride-sensitive Na+ channel, BLINaC, HINAC, ACCN5
    Cat #: ASC-032
    Alternative Name Acid-sensing ion channel 5, Amiloride-sensitive cation channel 5, Brain-liver-intestine amiloride-sensitive Na+ channel, BLINaC, HINAC, ACCN5
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
    Immunogen
    • Peptide (C)RQLKTVHQEYPWGE, corresponding to amino acid residues 284-297 of mouse ASIC5 (Accession Q9R0Y1). Extracellular loop.
    • Anti-ASIC5 (extracellular) Antibody
    Accession (Uniprot) Number Q9R0Y1
    Gene ID 58170
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, human – 13/14 amino acid residues identical.
    RRID AB_2756792.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*, lci*
    Western blot
    • Anti-ASIC5 (extracellular) Antibody
      Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat small intestine (lanes 3 and 6) lysates:
      1-3. Anti-ASIC5 (extracellular) Antibody (#ASC-032), (1:200).
      4-6. Anti-ASIC5 (extracellular) Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-ASIC5 (extracellular) Antibody
      Expression of ASIC5 in mouse cortex
      Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ASIC5 (extracellular) Antibody (#ASC-032), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. ASIC5 immunoreactivity (green) is detected in cells of the pyramidal layer (arrows). Cell nuclei are stained with DAPI (blue).
    References
    1. Boiko, N. et al. (2014) PLoS One 9, e91326.
    2. Cheng, Y.R. et al. (2018) J. Biomed. Sci. 25, 46.
    3. Karsan, N. et al. (2018) Neurotherapeutics 15, 402.
    Scientific background

    ASIC5 (acid sensing ion channel subunit family member 5) is an amiloride-sensitive ion channel belonging to the Degenerin/Epithelial Na+ channel (Deg/ENaC) family, a group of voltage-insensitive cation channels expressed in the nervous system and in several types of epithelial and immune cells.

    Structurally, ASICs have two transmembrane domains which line the pore of the channel with a relatively short cytoplasmic section, intracellular N- and C-termini and a large extracellular loop. The proton ligand binds to the extracellular domain, composed of 12 β sheets and 7 α helices2,3.

    ASICs are permeable to sodium, potassium, and sometimes to calcium and are activated by low pH, both intracellular and extracellular. They are responsible for conducting depolarizing inward Na+ currents. ASICs also contribute to excitotoxic neuronal damage during ischemia and local acidosis due to their sensitivity to acid1-3.

    ASICs are abundantly expressed in mammalian neurons. ASIC5 is mainly expressed in the brain in the cerebellum, specifically in the ventral uvula and nodulus of the vestibulocerebellum. ASIC5 mRNA is also detected in the liver mainly in the luminal membrane of epithelial cholangiocytes that line the bile duct1.

    ASICs have been linked to numerous neurological diseases including Parkinson’s disease, stroke, traumatic brain injury, multiple sclerosis, and epilepsy3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-ASIC5 (extracellular) Antibody (#ASC-032) is a highly specific antibody directed against an epitope of the mouse protein. It can be used in western blot and immunohistochemistry applications. It has been designed to recognize ASIC5 from human, mouse, and rat samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Shipping and Ordering information