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Home › Products › Neural Signaling › PDZ-Domain Scaffolding Proteins › Antibodies

Certificate of Analysis

Anti-CASK Antibody

Peripheral plasma membrane protein CASK, Calcium/calmodulin-dependent serine protein kinase, Membrane-associated guanylate kinase, CMG, CAMGUK, LIN2, TNRC8

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Overview

Cat #: AIP-018
Alternative Name Peripheral plasma membrane protein CASK, Calcium/calmodulin-dependent serine protein kinase, Membrane-associated guanylate kinase, CMG, CAMGUK, LIN2, TNRC8
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KERDRYAYKIHLPE, corresponding to amino acid residues 277-290 of mouse CASK (Accession O70589). Intracellular.
Accession (Uniprot) Number O70589
Gene ID 12361
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, human – identical.
RRID AB_2756656.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Rat and mouse brain lysates. Human brain neuroblastoma (SH-SY5Y) cell line lysates (1:200-1:2000).
  • Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and human neuroblastoma (SH-SY5Y) (lanes 3 and 6) cell line lysates:
    Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and human neuroblastoma (SH-SY5Y) (lanes 3 and 6) cell line lysates:
    1,2. Anti-CASK Antibody (#AIP-018), (1:400).
    3. Anti-CASK Antibody (1:200).
    4,5,6. Anti-CASK Antibody, preincubated with CASK Blocking Peptide (#BLP-IP018).
Immunohistochemistry
  • Rat brain sections (1:200).
Scientific background

The CASK protein belongs to the membrane-associated guanylate kinase family of proteins (MAGUK). MAGUK proteins generally target neuronal synapses and regulate trafficking, targeting, and signaling of ion channels (like CaV channels).

CASK consists of the following modular domains- the PDZ, Src homolog 3 (SH3), and guanylate kinase (GK) domains that are the characteristic protein domains shared by all MAGUK proteins. In addition, CASK contains two L27 domains preceding the single PDZ domain and has a unique CaMK-like domain at its N-terminal region.

The PDZ domain, consisting of approximately 90 amino acid residues, is specialized for binding to short peptide motifs at the extreme C-terminal tail of its binding partners, although other modes of interaction also occur. The Src homolog 3 (SH3) domain typically interacts with a proline-rich sequence. In addition to interaction with the proline-rich motif, the CASK SH3 domain mediates interaction with the GK domain. This interaction can occur intra- or intermolecularly. CASK protein can therefore form a homodimer via the inter-molecular SH3-GK interaction. Because the CASK SH3 domain also recognizes the GK domain of other MAGUK proteins, such as p55, CASK also forms heterodimers with other MAGUK proteins via an intermolecular SH3-GK interaction. The CASK CaMK-like domain functions as a protein-protein interacting domain, which binds to several cellular proteins, such as Mint1/X11, Caskin 1, CIP98, and Carom.

CASK is mostly expressed in (but not restricted to) neurons; expression levels of CASK are 3-5-fold higher in the brain than in other organs. In addition to playing an important role in the nervous system, CASK has also been suggested as having a role in establishing epithelial cell polarity in mammals1.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
For research purposes only, not for human use
Last Update: 10/04/2023

Specifications

Citations

Citations

Applications

Scientific Background

Specific Control Product

  • CASK Blocking Peptide (#BLP-IP018) is the original antigen used for immunization during Anti-CASK Antibody (#AIP-018) generation. The blocking peptide binds and ‘blocks’ Anti-CASK primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

    CASK Blocking Peptide (#BLP-IP018)

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Antibodies

  1. Anti-PSD-95 Antibody (#APZ-009)
  2. Anti-PICK1 Antibody (#APZ-014)
  3. Anti-NHERF-1/EBP50 Antibody (#APZ-006)
  4. Anti-Shank3 Antibody (#APZ-013)
  5. Anti-MAGI2/AIP1 Antibody (#APZ-052)

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