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Anti-EphA6 (extracellular) Antibody

Ephrin type-A receptor 6, EPH receptor A6, EPH homology kinase 2, EHK-2

Cat #: AER-016
Alternative Name Ephrin type-A receptor 6, EPH receptor A6, EPH homology kinase 2, EHK-2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KEHEQLTYSSTRSK, corresponding to amino acid residues 482-495 of rat EphA6 (Accession P54758). Extracellular, N-terminus.
Accession (Uniprot) Number P54758
Gene ID 29202
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
RRID AB_2876801
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    1, 2. Anti-EphA6 (extracellular) Antibody (#AER-016), (1:200).
    3, 4. Anti-EphA6 (extracellular) Antibody, preincubated with EphA6 (extracellular) Blocking Peptide (#BLP-ER016).
  • Western blot analysis of human LNCaP prostate adenocarcinoma cell line lysate:
    Western blot analysis of human LNCaP prostate adenocarcinoma cell line lysate:
    1. Anti-EphA6 (extracellular) Antibody (#AER-016), (1:400).
    2. Anti-EphA6 (extracellular) Antibody, preincubated with EphA6 (extracellular) Blocking Peptide (#BLP-ER016).
Immunohistochemistry
  • Expression of EphA6 in rat parietal cortex
    Expression of EphA6 in rat parietal cortex
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-EphA6 (extracellular) Antibody (#AER-016), (1:200), followed by goat anti-rabbit-AlexaFluor-488. EphA6 immunoreactivity (green) appears in pyramidal cells (arrows). Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of EphA6 in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of EphA6 in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-EphA6 (extracellular) Antibody (#AER-016), (2.5 µg) + goat-anti-rabbit-FITC.
References
  1. Zhou, D. et al. (2018) Oncol. Lett. 15, 5257.
  2. Das, G. et al. (2016) Cell Biosci. 6, 48.
  3. Teng, S. et al. (2019) Brain Inj. 33, 1385.
  4. Darling, T.K. et al. (2019) Front. Immunol. 10, 1473.
Scientific background

EphA6 is an erythropoietin-producing hepatocellular (Eph) receptor that belongs to the tyrosine kinase receptor family. The Eph receptor family consists of 9 EphA receptors and 5 EphB receptors, which serve key roles in normal physiology, including cell migration, cell adhesion, cell proliferation and neuronal synaptic plasticity. These activities depend on the binding of their Eph receptor-interacting (ephrin) proteins, of which include 8 members. Compelling data has demonstrated that Eph receptors and ephrins affect tumor growth, invasiveness, angiogenesis and metastasis1. They are also involved in brain development, learning and memory2.

EphA6 signaling pathways are inhibitory for developing axons and blocking these pathways enhances regeneration following spinal cord and brain injury; rendering EphA6 an appealing drug target3.

Eph receptors contain an extracellular structure consisting of an ephrin binding domain, the juxtamembrane region connects the extracellular portion of the receptor to the intracellular kinase domain. Two Tyrosine residues on the juxtamembrane region mediate autophosphorylation and receptor dimerization mediates the formation of heterocomplexes that are required for signaling4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 27/06/2021

Anti-EphA6 (extracellular) Antibody (#AER-016) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect live cell flow cytometry, and immunohistochemistry applications. It has been designed to recognize EphA6 from rat, mouse, and human samples.

For research purposes only, not for human use
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