Free shipping starts now, no minimum, no coupons required!

Anti-EphA2 (extracellular) Antibody

Ephrin type A receptor 2, EPH Receptor A2, Epithelial Cell Kinase, Tyrosine-Protein Kinase Receptor ECK, ECK, EPHA2
Cat #: AER-019
Alternative Name Ephrin type A receptor 2, EPH Receptor A2, Epithelial Cell Kinase, Tyrosine-Protein Kinase Receptor ECK, ECK, EPHA2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)HYLTAVGMGAKVELR, corresponding to amino acid residues 333 - 347 of human EphA2 (Accession P29317). Extracellular, N-terminus.
Accession (Uniprot) Number P29317
Gene ID 1969
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat – 14/15 amino acid residues identical; mouse – 13/15 amino acid residues identical.
RRID AB_2876848
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, wb
May also work in: ic*, ih*, ip*, lci*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
    1, 2. Anti-EphA2 (extracellular) Antibody (#AER-019), (1:200).
    3, 4. Anti-EphA2 (extracellular) Antibody, preincubated with EphA2 (extracellular) Blocking Peptide (#BLP-ER019).
  • Western blot analysis of human U87-MG glioblastoma cell line lysate (lanes 1 and 5), human THP-1 monocytic leukemia cell line lysate (lanes 2 and 6), human LNCaP prostate adenocarcinoma cell line lysate (lanes 3 and 7) and human MCF-7 breast adenocarcinoma cell line lysate (lanes 4 and 8):
    Western blot analysis of human U87-MG glioblastoma cell line lysate (lanes 1 and 5), human THP-1 monocytic leukemia cell line lysate (lanes 2 and 6), human LNCaP prostate adenocarcinoma cell line lysate (lanes 3 and 7) and human MCF-7 breast adenocarcinoma cell line lysate (lanes 4 and 8):
    1-4. Anti-EphA2 (extracellular) Antibody (#AER-019), (1:200).
    5-8. Anti-EphA2 (extracellular) Antibody, preincubated with EphA2 (extracellular) Blocking Peptide (#BLP-ER019).
Indirect flow cytometry
  • Cell surface detection of EphA2 in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of EphA2 in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-EphA2 (extracellular) Antibody (#AER-019), (2.5 µg) + goat-anti-rabbit-FITC.
References
  1. Pasquale, E.B. (2008) Cell 133, 38.
  2. Zhou, Y. and Sakurai, H. (2017) Biol. Pharm. Bull. 40, 1616.
  3. Wykosky, J. and Debinski, W. (2008) Mol. Cancer. Res. 6, 1795.
  4. Tandon, M. et al. (2011) Expert Opin. Ther. Targets 15, 31.
Scientific background

Erythropoietin-producing hepatocellular receptor A2 (EphA2) is a tyrosine kinase that belongs to the Ephrin (Eph) family of receptors. Members bind to cell surface-associated Ephrin ligands on neighboring cells. This binding generates bidirectional signaling which is a major form of contact-dependent communication between cells1

Eph receptors are divided into two classes, EphA and EphB, depending on the homology of their extracellular domains. Each extracellular domain is composed of a ligand-binding domain, Sushi domain, epidermal growth factor (EGF)-like domain, and two fibronectin type-III repeats. Eph receptors also contain a transmembrane domain, juxtamembrane region, tyrosine kinase domain, sterile alpha motif (SAM) and postsynaptic density (PSD)-95 protein, Discs large, and Zona occludens tight junction protein (PDZ) domain-binding motif.

The interaction between Ephrin and the Eph receptor results in both “forward signaling” and “reverse signaling,” which are mediated by Eph receptor and Ephrin, respectively2. Forward signaling induces Eph receptor oligomer clustering, cross-phosphorylation of tyrosine residues on the juxtamembrane domain and activation loop and, consequently, activation of the kinase activity.

Eph receptor-ligand interactions mainly regulate cell proliferation and migration during development as well as tissue homeostasis, axon guidance, synapse plasticity, tissue remodeling, bone morphogenesis, and angiogenesis2.

EphA2 is expressed in normal epidermal cells and in various solid tumors, such as breast, ovary, prostate, pancreas, glioblastoma, neck, renal, lung, melanoma, bladder and other types of cancer2.

EphA2 expression in tumors is associated with a more aggressive cancer phenotype and correlates with tumor metastasis and poor patient outcome. In contrast, EphA2 also reduces cancer cell motility and proliferation and hence has both pro- and anti-oncogenic roles, depending on the cellular context3.

Due to its pivotal role in various diseases, EphA2 is an appealing drug target and research is performed in order to control its activity4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Widget not in any sidebars
Last update: 20/10/2020

Anti-EphA2 (extracellular) Antibody (#AER-019) is a highly specific antibody directed against an epitope of human EPHA2. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize EphA2 from rat, mouse, and human samples.

For research purposes only, not for human use
Shipping and Ordering information