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Anti-EphB1 (extracellular) Antibody

Ephrin type-B receptor 1, Eph receptor B1, Tyrosine-protein kinase receptor EPH-2

Cat #: AER-021
Alternative Name Ephrin type-B receptor 1, Eph receptor B1, Tyrosine-protein kinase receptor EPH-2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
  • Peptide (C)RSQTNTARIDGLR, corresponding to amino acid residues 485-497 of mouse EphB1 (Accession Q8CBF3). Extracellular, N-terminus.
Accession (Uniprot) Number Q8CBF3
Gene ID 270190
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, human - identical.
RRID AB_2340932.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, lci, wb
May also work in: ih*, ip*
Western blot
  • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
    1,2. Anti-EphB1 (extracellular) Antibody (#AER-021), (1:800).
    3,4. Anti-EphB1 (extracellular) Antibody, preincubated with EphB1 (extracellular) Blocking Peptide (#BLP-ER021).
Indirect flow cytometry
  • Cell surface detection of EphB1 in live intact human THP-1 monocytic leukemia cell line:
    Cell surface detection of EphB1 in live intact human THP-1 monocytic leukemia cell line:
    ___ Cells + goat anti-rabbit-AlexaFluor-488 secondary antibody.
    ___ Cells + Anti-EphB1 (extracellular) Antibody (#AER-021), (1:15) + goat-anti-rabbit-Alexa-488.
Live cell imaging / Immunocytochemistry
  • Expression of EphB1 in rat PC12 cells
    Expression of EphB1 in rat PC12 cells
    Cell surface detection of EphB1 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-EphB1 (extracellular) Antibody (#AER-021), (1:100), followed by goat anti-rabbit-AlexaFluor-594 (red). B. Live image of the cells. C. Merge of the two images.
  1. Chen, Y. et al. (2012) Cell. Signal. 24, 606.
  2. Pasquale, E.B. (2008) Cell 133, 38.
  3. Pasquale, E.B. (2005) Mol. Cell Biol. 6, 462.
  4. Du, J. et al. (2007) Curr. Pharm. Des. 13, 2507.
  5. Wu, J. and Luo, H. (2005) Curr. Opin. Hematol. 12, 292.
  6. Ireton, R.C. and Chen, J. (2005) Curr. Cancer Drug Targets 5, 149.
  7. Noren, N.K. and Pasquale, E.B. (2007) Cancer Res. 67, 3994.
Scientific background

Eph receptors are the largest family of receptor tyrosine kinases (RTKs). EphA receptors (EphA1-10) bind ephrinA ligands which are GPI-linked proteins and EphB receptors (EphB1-6) bind ephrinB ligands which are membrane protein with one transmembrane domain1,2. Within each subfamily, interactions between receptor and ligand are promiscuous. In addition, Eph receptors can also bind ephrins from the other class2. Forward and reverse signaling through Eph receptors is a unique characteristic to this RTK since ephrins are physically linked to the plasma membrane3.

Structurally, Eph receptors contain an extracellular ligand-binding domain, a transmembrane domain and an intracellular C-terminal domain responsible for intracellular signaling1. Forward Eph receptor signaling involves autophosphorylation of the receptor via a tyrosine kinase domain, as well as phosphorylation of other proteins. Known effectors of the forward signaling include Src kinase and Ras/Rho GTPases2. Much less is known about the reverse signaling mediated by Eph receptors. Besides from acting independently, Eph receptors can also signal in concert with other receptors. For example, Eph receptors cooperate with FGF receptor, NMDA ligand-gated ion channel and chemokine G-protein coupled receptor2.

Biological activities attributed to the Eph receptor-ephrin signaling module include establishing neuronal connections, mediating neuronal plasticity and repair following neuronal injury2,4. Eph receptors may also have a role in the immune system5.

Eph receptors are expressed in the developing nervous system, and in the adult brain. It is also detected in the pancreas, intestine, bone and lymphocytes. In cancer cells, Eph receptors and ephrins are overexpressed2,6,7. They are also implicated in neurodegenerative disorders like Alzheimer’s disease1.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/01/2023

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of mouse EphB1. Anti-EphB1 (extracellular) Antibody (#AER-021) can be used in western blot, immunocytochemistry and indirect flow cytometry applications. The antibody is suitable for live cell imaging. It has been designed to recognize EphB1 from rat, mouse, and human samples.

For research purposes only, not for human use
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