Anti-Excitatory Amino Acid Transporter 5 (extracellular)

EAAT5, Retinal glutamate transporter, SLC1A7
    Cat #: AGC-025
    Sizes: 25 µl, 50 µl, 0.2 ml
  • Lyophilized Powder
  • Antigen Incl.
  • QC Tested
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: m, r
    May also work in: h*
    Immunogen
    Peptide (C)KETTEQSGKPVMS, corresponding to amino acid residues 122-134 of mouse EAAT5 (Accession Q8JZR4). 2nd extracellular loop.
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat – identical; human – 12/13 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ifc*, ic*, ih*, lci*, ip*
    Western blot
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysate:
    1, 2. Anti-Excitatory Amino Acid Transporter 5 (extracellular) antibody (#AGC-025), (1:200).
    3, 4. Anti-Excitatory Amino Acid Transporter 5 (extracellular), preincubated with the control peptide antigen.
    References
    1. Scopelliti, A.J. et al. (2013) J. Biol. Chem. 288, 8250.
    2. Benarroch, E.E. (2010) Neurology 74, 259.
    3. Nelson, P.T. et al. (2013) Acta Neuropathol. 126, 161.
    Scientific background

    Glutamate transporters play important roles in the termination of excitatory neurotransmission and in providing cells throughout the body with glutamate for metabolic purposes.

    SLC1A7 (EAAT5) belongs to The SLC1 protein family which contains a range of human and prokaryotic glutamate and aspartate transporters1. EAAT5 is expressed mainly in photoreceptors and bipolar cells in the retina and is essential for the maintenance of normal excitatory synaptic transmission as it determines the time course of glutamate receptor activation. EAAT5 consists of 3 identical subunits that are functionally coupled to the sodium (Na+), potassium (K+)-ATPase as part of the same macromolecular complex. The transporter operates via an “alternate access mechanism” whereby the translocation of one molecule of glutamate into the cell is coupled with the inward cotransport with 3Na+ and 1H+ ions. In turn, the return of the empty carrier to the extracellular side of the plasma membrane is coupled to the export of 1K+ ion. In addition, EAAT5, may function as glutamate-gated chloride (Cl) channel, generating an anion current that is not stoichiometrically coupled to the uptake of glutamate2.

    EAAT5 has been linked to the development of hippocampal sclerosis. Hippocampal sclerosis of aging (HS-Aging) is a causative factor in a large proportion of elderly dementia cases3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 25/12/2017

    Anti-Excitatory Amino Acid Transporter 5 (extracellular) (#AGC-025) is a highly selective antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope, and could potentially be used for detecting the protein in living cells. It has been designed to recognize EAAT5 from mouse, human, and rat samples.

    For research purposes only, not for human use