Overview
Alomone Labs is pleased to offer the Glutamate Transporter Antibody Explorer Kit (#AK-440). The Explorer Kit contains glutamate transporter antibodies, ideal for screening purposes.
Compounds
Compounds
| Product Name | Cat # | Size |
|---|---|---|
Anti-EAAT1 (GLAST) (extracellular) Antibody |
AGC-021 | 1 x 50 µl |
Anti-EAAT2 (GLT-1) (extracellular) Antibody |
AGC-022 | 1 x 50 µl |
Anti-EAAT3 (EAAC1) Antibody |
AGC-023 | 1 x 50 µl |
Anti-EAAT4 (extracellular) Antibody |
AGC-024 | 1 x 50 µl |
Anti-SLC1A7 (extracellular) Antibody |
AGC-025 | 1 x 50 µl |
Anti-VGLUT1 Antibody |
AGC-035 | 1 x 50 µl |
Anti-VGLUT2 Antibody |
AGC-036 | 1 x 50 µl |
Anti-VGLUT3 Antibody |
AGC-037 | 1 x 50 µl |
Scientific Background
Scientific Background
The transport of Glu into the cells by the EAAT transporters is coupled to the Na+ and K+ electrochemical gradient as a driving force. Hence, the uptake of Glu is dependent on the co-transport of three Na+ and one H+ ions, and the counter transport of one K+ ion.
In addition, to the well documented Glu uptake, the EAAT transporters show a Glu-independent Cl- conductance. The physiological significance of the Cl- current through the EAATs is currently unknown.1,2
EAAT1 as well as EAAT2, is expressed predominantly in glia cells (hence its original name: glial glutamate transporter or GLAST), while EAAT3, EAAT4 and EAAT5 are mostly expressed in neurons.
Three vesicular glutamate transporters (VGLUTs) have been cloned: BNPI/VGLUT1 (a brain-specific sodium dependent inorganic phosphate (Pi) transporter), and its homologs DNPI/VGLUT2 (differentiation-associated sodium-dependent Pi transporter) and VGLUT33. These transporters mediate glutamate uptake inside presynaptic vesicles and are anatomical and functional markers of glutamatergic excitatory transmission4.
- Tzingounis, A.V. and Wadiche, J.I. (2007) Nat. Rev. Neurosci. 8, 935.
- Beart, P.M. and O’Shea, R.D. (2007) Br. J. Pharmacol. 150, 5.
- Gong, J. et al. (2006) Brain Res. 1082, 73.
- Fremeau, R.T. et al. (2004) Science 304, 1815.
