Anti-GRID2 (extracellular) Antibody

Ionotropic glutamate receptor δ2, GluR delta-2 subunit, GluD2
Cat #: AGC-039
Alternative Name Ionotropic glutamate receptor δ2, GluR delta-2 subunit, GluD2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide (C)KMITTLFDTMRIE, corresponding to amino acid residues 206-218 of rat GluD2 (Accession Q63226). Extracellular, N-terminus.
    Accession (Uniprot) Number Q63226
    Gene ID 79220
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human, mouse - identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, lci, wb
    May also work in: ifc, ip
      • Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human U87-MG glioblastoma cell lysate (lines 3 and 6):
        1-3. Anti-GRID2 (extracellular) Antibody (#AGC-039), (1:600).
        4-6. Anti-GRID2 (extracellular) Antibody, preincubated with the control peptide antigen.
      • Mouse brain sections (1:200).
        Pernice, H.F. et al. (2019) Int. J. Mol. Sci. 20, 1797.
      • Expression of Glutamate receptor δ2 in rat PC12 cells
        Cell surface detection of GluD2 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-GRID2 (extracellular) Antibody (#AGC-039), (1:50), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of A and B. Cell nuclei (blue) were visualized with Hoechst 33342.
      • Excitatory neurotransmission in the vertebrate central nervous system is mainly mediated by ionotropic glutamate receptors (iGluRs). Molecular cloning identified 18 mammalian iGluR subunits, of which only 16 sort into the traditional pharmacological subfamilies of AMPAkainate (KA), and N-methyl-D-aspartate (NMDA) receptors. The 2 remaining subunits were termed “orphan” receptors, “glutamate-like” receptors, “nonionotropic” receptors, or, most commonly, delta receptors1.

        Ionotropic glutamate receptors are integral membrane proteins composed of four large subunits that form a central ion channel pore. Sequence similarity among all known glutamate receptor subunits, including the δ receptors, suggests they share a similar architecture. Glutamate receptor subunits are modular structures that contain four discrete semiautonomous domains: the extracellular amino-terminal domain (ATD), the extracellular ligand-binding domain (LBD), the transmembrane domain (TMD), and an intracellular carboxyl-terminal domain (CTD)2

        The delta family of ionotropic glutamate receptors (iGluRs) consists of the glutamate δ1 (GluD1) and glutamate δ2 (GluD2) receptors3. GluD1 is highly expressed in the inner hair cells of the organ of Corti4, diffusely expressed throughout the forebrain during development with high levels in the hippocampus during adulthood3. Deletion of GluD1 leads to a deficit in high frequency hearing in mice5. Genetic association studies have established the GRID1 gene, which codes for GluD1, is a strong candidate gene for schizophrenia, bipolar disorder, and major depressive disorder6. Copy number variation studies have also implicated GRID1 in autism spectrum disorder (ASD)7. In addition, GRID1 gene is localized to the 10q22-q23 genomic region which is a site for recurrent deletions associated with cognitive and behavioral abnormalities8.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 18/06/2019

    Anti-GRID2 (extracellular) Antibody (#AGC-039) is a highly specific antibody directed against an epitope of the rat Glutamate receptor δ2. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize GluD2 from rat, mouse, and human samples.

    For research purposes only, not for human use