- Peptide (C)KNKTEAFALEKFYR, corresponding to amino acid residues 355-368 of rat GlyRα3 (Accession P24524). 2nd intracellular loop.
- Western blot analysis of rat brain membranes:1. Anti-Glycine Receptor α3 Antibody (#AGR-003), (1:200).
2. Anti-Glycine Receptor α3 Antibody, preincubated with Glycine Receptor α3 Blocking Peptide (#BLP-GR003).
- Rat trigeminal ganglion sections (1:100) (Bae, J.Y. et al. (2016) Brain Struct. Funct. 221, 4601.).
Glycine is a major neurotransmitter in the mammalian central nervous system.1 The glycine receptor is a ligand gated Cl- channel, mediating synaptic inhibition in the spinal cord, retina and regions of the brain.2
The glycine receptor is a pentamer consisting of α and β subunits. To date there are four known α-subunit isoforms (α1-α4) encoded by different genes.2-4
In humans, two alternative splice variants of the glycine receptor α3 have been described: α3L and α3K, where the α3L splice variant bears an additional segment of 15 amino acids within the long cytoplasmic TM3-4 loop. These variants differ in their desensitization kinetics.3
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat glycine receptor α3. Anti-Glycine Receptor α3 Antibody (#AGR-003) can be used in western blot and immunohistochemistry applications. It has been designed to recognize GlyRα3 from rat, mouse and human samples.