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Anti-Glycine Receptor α3 Antibody

Glycine receptor subunit alpha-3, GLRA3, GlyRα3

Cat #: AGR-003
Alternative Name Glycine receptor subunit alpha-3, GLRA3, GlyRα3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KNKTEAFALEKFYR, corresponding to amino acid residues 355-368 of rat GlyRα3 (Accession P24524). 2nd intracellular loop.
Accession (Uniprot) Number P24524
Gene ID 114516
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - identical.
RRID AB_2039889.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat brain membranes:
    Western blot analysis of rat brain membranes:
    1. Anti-Glycine Receptor α3 Antibody (#AGR-003), (1:200).  
    2. Anti-Glycine Receptor α3 Antibody, preincubated with Glycine Receptor α3 Blocking Peptide (#BLP-GR003).
Immunohistochemistry
  • Rat trigeminal ganglion sections (1:100) (Bae, J.Y. et al. (2016) Brain Struct. Funct. 221, 4601.).
References
  1. Becker, C.M. (1995) The Neuroscientist 1, 130.
  2. Gisselmann, G. et al. (2002) Eur. J. Neurosci. 16, 69.
  3. Nikolic, Z. et al. (1998) J. Biol. Chem. 273, 19708.
  4. Breitinger, H.G. et al. (2002) J.Neurochem. 83, 30.
Scientific background

Glycine is a major neurotransmitter in the mammalian central nervous system.1 The glycine receptor is a ligand gated Cl- channel, mediating synaptic inhibition in the spinal cord, retina and regions of the brain.2

The glycine receptor is a pentamer consisting of α and β subunits. To date there are four known α-subunit isoforms (α1-α4) encoded by different genes.2-4  

In humans, two alternative splice variants of the glycine receptor α3 have been described: α3L and α3K, where the α3L splice variant bears an additional segment of 15 amino acids within the long cytoplasmic TM3-4 loop. These variants differ in their desensitization kinetics.3

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
Last update: 22/05/2022

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat glycine receptor α3. Anti-Glycine Receptor α3 Antibody (#AGR-003) can be used in western blot and immunohistochemistry applications. It has been designed to recognize GlyRα3 from rat, mouse and human samples.

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For research purposes only, not for human use

Applications

Citations

Citations
Immunohistochemistry citations
  1. Rat trigeminal ganglion sections (1:100).
    Bae, J.Y. et al. (2016) Brain Struct. Funct. 221, 4601.

Specifications

Scientific Background

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