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Anti-Glycine Receptor β Antibody

Glycine receptor subunit beta, Glycine receptor 58 kDa subunit, GLRB, GlyRβ

Cat #: AGR-014
Alternative Name Glycine receptor subunit beta, Glycine receptor 58 kDa subunit, GLRB, GlyRβ
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide HISTLQVGETRC, corresponding to amino acid residues 394-405 of rat GlyRβ (Accession P20781). 2nd intracellular loop.
Accession (Uniprot) Number P20781
Gene ID 25456
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
RRID AB_2340973.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    1,2. Anti-Glycine Receptor β Antibody (#AGR-014), (1:200).
    3,4. Anti-Glycine Receptor β Antibody, preincubated with Glycine Receptor β Blocking Peptide (#BLP-GR014).
References
  1. Meyer, G. et al. (1995) Neuron 15, 563.
  2. Del Pino, I. et al. (2014) J Biol Chem. 289, 11396.
  3. Weltzien, F. et al. (2012) J. Comp. Neurol. 520, 3962.
  4. Al-Owain, M. et al. (2012) Clin. Genet. 81, 479.
  5. Mulhardt, C. et al. (1994) Neuron 13, 1003.
  6. Hirata, H. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 8345.
Scientific background

The amino acid glycine mediates neuronal inhibition by activating glycine receptors (GlyRs), which are ligand-gated chloride channels of the nicotinic acetylcholine receptor superfamily. GlyRs are known to mediate postsynaptic inhibition in spinal cord, brain stem and some higher brain regions1. GlyRs are members of the pentameric Cys-loop family of ionotropic neurotransmitter receptors.

GlyRs are composed of homologous α (α1-α4) and β subunits. Upon synthesis at the endoplasmic reticulum (ER), all GlyRα subunits can assemble into functional homopentamers that are inserted into the plasma membrane. In contrast, the GlyRβ subunit requires stoichiometric coassembly with GlyRα subunits for ER exit and cell surface expression. Each subunit consists of an extended N-terminal extracellular domain, four transmembrane domains, and a large intracellular loop between transmembrane domains 3 and 4. The β subunit is responsible for synaptic localization of the GlyRs by interacting with the postsynaptic scaffolding protein gephyrin. GlyRβ subunit is reported to interact with the proteins Vacuolar Protein Sorting 35 (Vps35) and Neurobeachin (Nbea)2.

GlyRβ mRNA is abundantly found throughout the late embryonic and postnatal brain3.

Mutations in GLRB, the gene encoding GlyRβ, have been discovered in patients with hyperekplexia4, in the mouse mutant spastic5 and the zebrafish bandoneon mutant6.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat glycine receptor β. Anti-Glycine Receptor β Antibody (#AGR-014) can be used in western blot analysis. It has been designed to recognize GlyRβ from human, rat and mouse samples.

For research purposes only, not for human use
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