- Peptide HISTLQVGETRC, corresponding to amino acid residues 394-405 of rat GlyRβ (Accession P20781). 2nd intracellular loop.
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-Glycine Receptor β Antibody (#AGR-014), (1:200).
3,4. Anti-Glycine Receptor β Antibody, preincubated with Glycine Receptor β Blocking Peptide (#BLP-GR014).
- Meyer, G. et al. (1995) Neuron 15, 563.
- Del Pino, I. et al. (2014) J Biol Chem. 289, 11396.
- Weltzien, F. et al. (2012) J. Comp. Neurol. 520, 3962.
- Al-Owain, M. et al. (2012) Clin. Genet. 81, 479.
- Mulhardt, C. et al. (1994) Neuron 13, 1003.
- Hirata, H. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 8345.
The amino acid glycine mediates neuronal inhibition by activating glycine receptors (GlyRs), which are ligand-gated chloride channels of the nicotinic acetylcholine receptor superfamily. GlyRs are known to mediate postsynaptic inhibition in spinal cord, brain stem and some higher brain regions1. GlyRs are members of the pentameric Cys-loop family of ionotropic neurotransmitter receptors.
GlyRs are composed of homologous α (α1-α4) and β subunits. Upon synthesis at the endoplasmic reticulum (ER), all GlyRα subunits can assemble into functional homopentamers that are inserted into the plasma membrane. In contrast, the GlyRβ subunit requires stoichiometric coassembly with GlyRα subunits for ER exit and cell surface expression. Each subunit consists of an extended N-terminal extracellular domain, four transmembrane domains, and a large intracellular loop between transmembrane domains 3 and 4. The β subunit is responsible for synaptic localization of the GlyRs by interacting with the postsynaptic scaffolding protein gephyrin. GlyRβ subunit is reported to interact with the proteins Vacuolar Protein Sorting 35 (Vps35) and Neurobeachin (Nbea)2.
GlyRβ mRNA is abundantly found throughout the late embryonic and postnatal brain3.
Mutations in GLRB, the gene encoding GlyRβ, have been discovered in patients with hyperekplexia4, in the mouse mutant spastic5 and the zebrafish bandoneon mutant6.
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat glycine receptor β. Anti-Glycine Receptor β Antibody (#AGR-014) can be used in western blot analysis. It has been designed to recognize GlyRβ from human, rat and mouse samples.
- Anti-Glycine Receptor α1 Antibody (#AGR-001)
- Anti-Glycine Receptor α3 Antibody (#AGR-003)
- Anti-Glycine Receptor α4 (extracellular) Antibody (#AGR-015)
- Anti-GlyT1 (SLC6A9) (extracellular) Antibody (#AGT-011)
- Anti-GlyT2 (SLC6A5) (extracellular) Antibody (#AGT-012)
- Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGT-005)
- Anti-Vesicular GABA Transporter (VGAT)-ATTO Fluor-594 Antibody (#AGT-005-AR)