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Anti-GPR37 (extracellular) Antibody

G-protein coupled receptor 37, Prosaposin receptor GPR37, Parkin-associated endothelin receptor-like receptor, Endothelin B receptor-like protein 1

Cat #: AGR-049
Alternative Name G-protein coupled receptor 37, Prosaposin receptor GPR37, Parkin-associated endothelin receptor-like receptor, Endothelin B receptor-like protein 1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RVRLKNPFYPLTQE, corresponding to amino acid residues 234-247 of rat GPR37 (Accession Q9QYC6). Extracellular, N-terminus.
Accession (Uniprot) Number Q9QYC6
Gene ID 117549
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, wb
May also work in: ic*, ih*, ip*, lci*
Western blot
  • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    1,2. Anti-GPR37 (extracellular) Antibody (#AGR-049), (1:200).
    3,4. Anti-GPR37 (extracellular) Antibody, preincubated with GPR37 (extracellular) Blocking Peptide (#BLP-GR049).
Indirect flow cytometry
  • Cell surface detection of GPR37 in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of GPR37 in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-GPR37 (extracellular) Antibody (#AGR-049), (2.5 µg) + goat-anti-rabbit-FITC.
References
  1. Morató, X. et al. (2017) Sci. Rep. 7, 9452.
  2. Berger, B.S. et al. (2017) EMBO Rep. 18, 712.
  3. Lopes, J.P. et al. (2015) J. Neurochem. 134, 135.
Scientific background

GPR37 or parkin-associated endothelin-like receptor, is an orphan G-protein coupled receptor (GPCR) almost exclusively expressed in the central nervous system. High levels of the protein are detected in the cerebellum, corpus callosum, caudate nucleus, putamen, substantia nigra, and hippocampus.

GPR37 is a substrate of the E3 ubiquitin ligase parkin and acts as a receptor for prosaposin. The protein affects oligodendrocyte differentiation and myelination during development1,2.

Studies show that GPR37 is heavily involved in the pathogenesis of Parkinson's disease. Missense mutations of GPR37 substrate E3 ubiquitin ligase parkin result in the accumulation of GPR37 in the brain of Parkinson's disease patients1,2. In addition, GPR37 levels are up‐regulated in the brains of juvenile Parkinson disease patients and down‐regulated in the amygdala and hippocampus of major depressive disorder patients3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 02/03/2021

Anti-GPR37 (extracellular) Antibody (#AGR-049) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR37 from human, rat and mouse samples.

For research purposes only, not for human use
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