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Anti-Histamine H1 Receptor/HRH1 (extracellular) Antibody

Histamine Receptor H1, H1R
Cat #: AHR-006
Alternative Name Histamine Receptor H1, H1R
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)REDK(S)ETDFYNVTWFK, corresponding to amino acid residues 176 - 191 of mouse H1 Histamine Receptor (Accession P70174). 2nd extracellular loop.
Accession (Uniprot) Number P70174
Gene ID 15465
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse and rat – 15/16 amino acid residues identical; human – 14/16 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ifc, ih, wb
May also work in: ic*, ip*, lci*
Western blot
  • Western blot analysis of mouse brain membranes (lanes 1 and 5), rat brain membranes (lanes 2 and 6), rat lung membranes (lanes 3 and 7) and mouse lung membranes (lanes 4 and 8):
    Western blot analysis of mouse brain membranes (lanes 1 and 5), rat brain membranes (lanes 2 and 6), rat lung membranes (lanes 3 and 7) and mouse lung membranes (lanes 4 and 8):
    1-4. Anti- Histamine H1 Receptor/HRH1 (extracellular) Antibody (#AHR-006), (1:200).
    5-8. Anti-Histamine H1 Receptor/HRH1 (extracellular) Antibody, preincubated with Histamine H1 Receptor/HRH1 (extracellular) Blocking Peptide (#BLP-HR006).
  • Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 4), human U-87 MG glioblastoma cell line lysate (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 6):
    Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 4), human U-87 MG glioblastoma cell line lysate (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 6):
    1-3. Anti- Histamine H1 Receptor/HRH1 Antibody (extracellular) (#AHR-006), (1:200).
    4-6. Anti-Histamine H1 Receptor/HRH1 (extracellular) Antibody, preincubated with Histamine H1 Receptor/HRH1 (extracellular) Blocking Peptide (#BLP-HR006).
Immunohistochemistry
  • Expression of Histamine H1 Receptor in mouse substantia nigra
    Expression of Histamine H1 Receptor in mouse substantia nigra
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Histamine H1 Receptor/HRH1 Antibody (extracellular) (#AHR-006), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Histamine H1 Receptor immunoreactivity (green) appears in neuronal profiles (vertical arrows). B. Pre-incubation of the antibody with Histamine H1 Receptor (HRH1) (extracellular) Blocking Peptide (#BLP-HR006), suppresses staining. Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of Histamine H1 Receptor in live intact human MEG-01 megakaryoblastic leukemia cells:
    Cell surface detection of Histamine H1 Receptor in live intact human MEG-01 megakaryoblastic leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-Histamine H1 Receptor/HRH1 (extracellular) Antibody (#AHR-006), (5 µg) + goat-anti-rabbit-FITC.
References
  1. Shiroishi, M. and Kobayashi, T. (2016) Handb. Exp. Pharmacol. 241, 21.
  2. Hill, S.J. (1990) Pharmacol. Rev. 42, 45.
  3. Obara, I. et al. (2020) Br. J. Pharmacol. 177, 580.
Scientific background

Histamine, acts via distinct histamine H1, H2, H3, and H4 receptors, and regulates various physiological and pathological processes. Hence the activity of Histamine receptors is very crucial. They are members of G-protein coupled receptors superfamily. Thus, have seven transmembrane domains, extracellular N-terminal tail and a cytoplasmic C-terminus1.

H1 receptor is excitatory and couples to Gq‐type proteins, activating a signaling cascade which eventually leads to an increased cellular concentration of Ca2+ 3.

H1 receptor is implicated in type I hypersensitivity allergic reactions caused by numerous allergens1. An allergic reaction occurs when allergens activate mast cells that release histamine, the released histamine binds to H1R and this causes an activation of a cascade that leads to vasodilation and vascular hyper-permeability, resulting in allergic inflammation.

H1R is expressed in various tissues throughout body, including the airway, vascular smooth muscle, and brain2. H1R expressed in the brain plays an important role in the regulation of sleep-arousal cycle and memory by binding histamine where it acts as a neurotransmitter1

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

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Last update: 18/10/2020

Anti-Histamine H1 Receptor/HRH1 (extracellular) Antibody (#AHR-006) is a highly specific antibody directed against an epitope of mouse H1R. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize H1R from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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