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Anti-Human ASCT2/SLC1A5 (extracellular)-FITC Antibody

Alanine/Serine/Cysteine Transporter 2, Neutral amino acid transporter B(0), ATB(0), Sodium-dependent Neutral Amino Acid Transporter Type 2

Cat #: ANT-083-F
Alternative Name Alanine/Serine/Cysteine Transporter 2, Neutral amino acid transporter B(0), ATB(0), Sodium-dependent Neutral Amino Acid Transporter Type 2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h
Immunogen
  • Peptide (C)RSYSTTYEERNITGTR, corresponding to amino acid residues 202 - 217 of human ASCT2 (Accession Q15758). 2nd extracellular loop.
Accession (Uniprot) Number Q15758
Gene ID 6510
Homology Human only. Not recommended for use with rat and mouse samples.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Label Fluorescein isothiocyanate (FITC)
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #ANT-083), and direct flow cytometry (labeled antibody).
Applications: fc, lci
Direct flow cytometry
  • Cell surface detection of ASCT2 by direct flow cytometry in live intact human Jurkat T-cell leukemia cells:
    Cell surface detection of ASCT2 by direct flow cytometry in live intact human Jurkat T-cell leukemia cells:
    ___ Cells.
    ___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
    ___ Cells + Anti-Human ASCT2/SLC1A5 (extracellular)-FITC Antibody (#ANT-083-F), (2.5µg).
  • Cell surface detection of ASCT2 by direct flow cytometry in live intact human K562 chronic myeloid leukemia cells:
    Cell surface detection of ASCT2 by direct flow cytometry in live intact human K562 chronic myeloid leukemia cells:
    ___ Cells.
    ___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
    ___ Cells + Anti-Human ASCT2/SLC1A5 (extracellular)-FITC Antibody (#ANT-083-F), (5µg).
  • Cell surface detection of ASCT2 by direct flow cytometry in live intact human HL-60 promyelocytic leukemia cells:
    Cell surface detection of ASCT2 by direct flow cytometry in live intact human HL-60 promyelocytic leukemia cells:
    ___ Cells.
    ___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
    ___ Cells + Anti-Human ASCT2/SLC1A5 (extracellular)-FITC Antibody (#ANT-083-F), (5µg).
References
  1. Kasai, N. et al. (2017) Am. J. Transl. Res. 9, 3399.
  2. Marshall, A.D. et al. (2017) Oncogenesis 6, e367.
  3. Dong, J. et al. (2017) Oncogenesis 6, e356.
Scientific background

ASCT2 is an alanine-serine-cysteine amino acid transporter and solute linked carrier family 1 member A5 (SLC1A5). It functions as an essential high-affinity glutamine transporter. It also functions as a Na+-dependent transporter for alanine, serine and cysteine. The transport process is electroneutral which involves the Na+-coupled influx of glutamine to the Na+-coupled efflux of another substrate such as alanine, serine or cysteine. ASCT2 is expressed various peripheral tissues such as the lung and kidney1,2. ASCT2 has an important contribution to the homeostasis of amino acid metabolism within tumor cells3.

ASCT2 seems to be up regulated in several cancerous tissues such as stomach, liver, kidney, breast, prostate, skin, colorectal, pancreatic, tongue and lung cancers. Studies have shown that ASCT2 is necessary for activating critical survival and cellular growth signaling cascades in cancer, which includes mTOR and ERK pathways.  It has been shown that knockdown of ASCT2 in prostate cancer cell line xenograft models significantly inhibit tumor growth and metastasis in vivo. Thus, ASCT2 is a potential cancer therapeutic target1,2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 27/04/2022

Anti-Human ASCT2/SLC1A5 (extracellular) Antibody (#ANT-083) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize SLC1A5 from human samples only. The antibody will not recognize rat or mouse ASCT2.

Anti-Human ASCT2/SLC1A5 (extracellular)-FITC Antibody (#ANT-083-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize SLC1A5 from human samples only. The antibody will not recognize rat or mouse ASCT2.

For research purposes only, not for human use
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