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- Peptide (C)RGSQAQDRKLSTKE, corresponding to amino acid residues 673-686 of human multidrug resistance protein 1 (Accession P08183). 3rd intracellular loop.
The antibody is specific for MDR1. It won’t cross-react with the highly homologous MDR3 protein.
- Human LNCaP prostate carcinoma, human HeLa cervix carcinoma, and human HepG2 liver carcinoma cell lysates (1:200-1:1000).
- Western blot analysis of human LNCaP prostate carcinoma (lanes 1 and 4), human HeLa cervix carcinoma (lanes 2 and 5) and human HepG2 liver carcinoma (lanes 3 and 6) cell line lysates:1-3. Anti-Human ABCB1/MDR1 Antibody (#AMT-021), (1:200).
4-6. Anti-Human ABCB1/MDR1 Antibody, preincubated with the negative control antigen.
Multidrug resistance protein 1 (MRP1, also known as P glycoprotein 1) is a member of a superfamily of proteins known as the ATP-binding cassette (ABC) transporters. This superfamiy is classified into seven subfamilies (A through G). Subfamily ABCC is composed of 12 proteins and includes MRP1 and is encoded by ABCC1.
MRP1 structure comprises five domains with two nucleotide binding domains (NBDs) that bind ATP, and 12 transmembrane domains.
MRP1 mediates the ATP-dependent efflux of a variety of solutes across membranes against a concentration gradient. These solutes include antineoplastic agents such as vincristine and doxorubicin, physiological organic anions such as the antioxidant GSH and the pro-inflammatory cysteinyl leukotriene C4, hydrophilic conjugated xenobiotic, various antibiotics, opiates, antiviral agents, citalopram, and statins. MRP1 is the only MRP-related protein with an acknowledged role in tumor multidrug resistance in clinical oncology. It also plays an important role in the efficacy and toxicity of drugs used for the treatment of nonmalignant diseasese1,2.
Knock-out mouse model of Abcc1−/− has shown that MRP1 can act as an important factor of drug disposition because of its presence in cells at the interface between many tissues. The protein also demonstrates chemoprotective properties1.
Evidence suggests that ABC transporters also have an important role in normal cellular biology including regulation of cell differentiation, proliferation, and survival, including apoptosis, tumor promotion and modulation of immune function2.