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Anti-IGF1R (extracellular) Antibody

Insulin-like growth factor 1 receptor, IGF-I receptor, CD221
Cat #: ANT-045
Alternative Name Insulin-like growth factor 1 receptor, IGF-I receptor, CD221
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RQEYRKYGGAKLNR, corresponding to amino acid residues 883-896 of rat IGF1R (Accession P24062). Extracellular, N-terminus (β chain).
Accession (Uniprot) Number P24062
Gene ID 25718
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, wb
May also work in: ic*, ip*, lci*
Western blot
  • Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
    Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
    1, 2. Anti-IGF1R (extracellular) Antibody (#ANT-045), (1:400).
    3, 4. Anti-IGF1R (extracellular) Antibody, preincubated with IGF1R (extracellular) Blocking Peptide (#BLP-NT045).
  • Western blot analysis of human MCF-7 breast adenocarcinoma cell line lysate (lanes 1 and 4), human U-87 MG glioblastoma cell line lysate (lanes 2 and 5) and human THP-1 monocytic leukemia cell line lysate (lanes 3 and 6):
    Western blot analysis of human MCF-7 breast adenocarcinoma cell line lysate (lanes 1 and 4), human U-87 MG glioblastoma cell line lysate (lanes 2 and 5) and human THP-1 monocytic leukemia cell line lysate (lanes 3 and 6):
    1-3. Anti-IGF1R (extracellular) Antibody (#ANT-045), (1:400).
    4-6. Anti-IGF1R (extracellular) Antibody, preincubated with IGF1R (extracellular) Blocking Peptide (#BLP-NT045).
Immunohistochemistry
  • Expression of IGF1R in rat cortex and hippocampus
    Expression of IGF1R in rat cortex and hippocampus
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-IGF1R (extracellular) Antibody (#ANT-045), (1:1200), followed by goat anti-rabbit-AlexaFluor-488. A. IGF1R staining (green) in the parietal cortex is detected in neurons of the pyramidal layer (arrows). B. IGF1R immunoreactivity (green) in the hippocampal CA1 region is observed neurons of the pyramidal layer (arrows). Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of IGF1R in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of IGF1R in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-IGF1R (extracellular) Antibody (#ANT-045), (2.5 µg) + goat-anti-rabbit-FITC.
References
  1. Werner, H. et al. (2019) Expert Opin. Investig. Drugs 28, 1101.
  2. Chughtai, S. (2019) Cell Biochem. Funct. doi. 10.1002/cbf.3479.
  3. Li, J. et al. (2019) Nat. Commun. 10, 4567.
Scientific background

Insulin-like growth factor receptor (IGF1R) is a cell surface, tyrosine kinase receptor that can be activated by IGF1, IGF2, and insulin1.

IGF receptors are tyrosine kinase-containing heterotetramers that are linked to numerous cytoplasmic signaling cascades and have important roles in various cellular functions. IGF1R displays potent anti-apoptotic, pro-survival capacities and plays a key role in malignant transformation by regulating MAPK and PI3K/AKT signaling pathways. IGF1R regulators are identified as a candidate therapeutic targets in cancer and growth-related diseases1,2.

IGF1R and insulin receptor (IR) are closely related, and share 57% sequence identity and high structural similarity. Each IGF1R and IR receptor consists of the L1, CR, L2, FnIII-1,-2,-3, transmembrane (TM), and kinase domain. Two such protomers are linked by multiple disulfide bonds, forming a stable, covalent dimer. Upon ligand binding, the dimer undergoes significant conformational changes which facilitate its further signal transduction cascade3

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-IGF1R (extracellular) Antibody (#ANT-045) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize IGF1R from mouse, rat, and human samples.

For research purposes only, not for human use
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