- Peptide ENELSGWDYDYGFC, corresponding to amino acid residues 327-340 of rat LHCGR (Accession P16235). Extracellular, N-terminus.
- Western blot analysis of rat ovary lysate:1. Anti-LH Receptor (LHCGR) (extracellular) Antibody (#ALR-010), (1:2000).
2. Anti-LH Receptor (LHCGR) (extracellular) Antibody, preincubated with LH Receptor/LHCGR (extracellular) Blocking Peptide (#BLP-LR010).
- Expression of LHR in human ovary cell lineCell surface detection of LHR in intact living OVCAR3 cells. A. Extracellular staining of cells with Anti-LH Receptor (LHCGR) (extracellular) Antibody (#ALR-010), (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Nuclear staining of cells using DAPI as the counterstain. C. Merged images of A and B.
The luteinizing hormone receptor (LHR) is a member of the subfamily of glycoprotein hormone receptors within the superfamily of G-protein coupled receptor (GPCR)/seven-transmembrane domain receptors1. The LH/CG receptor is comprised of two structurally and functionally distinct domains, extracellular N-terminal exodomain and membrane-embedded endodomain. These two domains can separately be expressed and processed, including folding. The exodomain alone has the high-affinity hormone binding site but is not capable of generating hormonal signal. In contrast, the endodomain alone has the site for receptor activation2.
For many years, LHR was thought to be localized strictly to gonadal cells. In the testes, the LHR is thought to be restricted to Leydig cells. In the ovary, expression of the LHR occurs in theca cells, interstitial cells, differentiated granulosa cells, and luteal cells3. There is increasing evidence that LHR may be present in extragonadal tissues such as: uterus, female reproductive tract tissues, sperm, seminal vesicles, prostate, skin, breast cell lines, lactating mammary gland, adrenals, neural retina, neuroendocrine cells, and brain4.
Mutations in the LH receptor have a strong impact on the male phenotype. Activating mutations of the LH receptor leads to male-limited pseudoprecocious puberty, whereas inactivating mutations result in male pseudohermaphroditism5.
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat Luteinizing hormone receptor. Anti-LH Receptor (LHCGR) (extracellular) Antibody (#ALR-010) can be used in western blot and live cell imaging applications. It has been designed to recognize LHR from rat, mouse and human samples.