Anti-LH Receptor (extracellular) Antibody

Luteinizing hormone receptor, Lutropin-choriogonadotropic hormone receptor, LHR, LSH-R
    Cat #: ALR-010
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, r
    May also work in: m
    Immunogen
    Peptide ENELSGWDYDYGFC, corresponding to amino acid residues 327-340 of rat LH receptor (Accession P16235). Extracellular, N-terminus.
    Accession (Uniprot) Number P16235
    Gene ID 25477
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 13/14 amino acid residues identical; human - 12/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, lci, wb
    May also work in: ifc, ih, ip
    Western blot
    Western blot analysis of rat ovary lysate:
    1. Anti-LH Receptor (extracellular) Antibody (#ALR-010), (1:2000).
    2. Anti-LH Receptor (extracellular) Antibody, preincubated with the control antigen.
    Live cell imaging / Immunocytochemistry
    Expression of LH receptor in human ovary cell line
    Immunocytochemical staining of intact living OVCAR3 cells. A. Extracellular staining of cells with Anti-LH Receptor (extracellular) Antibody (#ALR-010), (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Nuclear staining of cells using DAPI as the counterstain. C. Merged images of A and B.
    References
    1. Dufau, M.L. et al. (1998) Annu. Rev. Physiol. 60, 461.
    2. Ji, T.H. et al. (1997) Recent Prog. Horm. Res52, 431.
    3. Ascoli, M. et al. (2002) Endocr. Rev. 23, 141.
    4. Rao, C.V. et al. (1996) J. Physiol. Pharmacol. 47, 41.
    5. Simoni, M. et al. (1997) Endocr. Rev. 18, 739.
    Scientific background

    The luteinizing hormone receptor (LHR) is a member of the subfamily of glycoprotein hormone receptors within the superfamily of G-protein coupled receptor (GPCR)/seven-transmembrane domain receptors1. The LH/CG receptor is comprised of two structurally and functionally distinct domains, extracellular N-terminal exodomain and membrane-embedded endodomain. These two domains can separately be expressed and processed, including folding. The exodomain alone has the high-affinity hormone binding site but is not capable of generating hormonal signal. In contrast, the endodomain alone has the site for receptor activation2.

    For many years, LHR was thought to be localized strictly to gonadal cells. In the testes, the LHR is thought to be restricted to Leydig cells. In the ovary, expression of the LHR occurs in theca cells, interstitial cells, differentiated granulosa cells, and luteal cells3. There is increasing evidence that LHR may be present in extragonadal tissues such as:  uterus, female reproductive tract tissues, sperm, seminal vesicles, prostate, skin, breast cell lines, lactating mammary gland, adrenals, neural retina, neuroendocrine cells, and brain4.

    Mutations in the LH receptor have a strong impact on the male phenotype. Activating mutations of the LH receptor leads to male-limited pseudoprecocious puberty, whereas inactivating mutations result in male pseudohermaphroditism5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 10/12/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat Luteinizing hormone receptor. Anti-LH Receptor (extracellular) Antibody (#ALR-010) can be used in western blot and live cell imaging applications. It has been designed to recognize LH receptor from rat, mouse and human samples.

    For research purposes only, not for human use